In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria (Apocynaceae) Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic Study.

Autor: Tekwu EM; Laboratory for Tuberculosis Research and Pharmacology, Biotechnology Centre, Nkolbisson, University of Yaoundé 1, Yaoundé, Cameroon; Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana., Bosompem KM; Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana., Anyan WK; Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana., Appiah-Opong R; Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana., Owusu KB; Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana., Tettey MD; Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana., Kissi FA; Noguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, Ghana., Appiah AA; Centre for Plant Medicine Research (CPMR), Akwapim, Mampong, Ghana., Penlap Beng V; Laboratory for Tuberculosis Research and Pharmacology, Biotechnology Centre, Nkolbisson, University of Yaoundé 1, Yaoundé, Cameroon., Nyarko AK; School of Pharmacy, College of Health Sciences, University of Ghana, Accra, Ghana.
Jazyk: angličtina
Zdroj: Journal of parasitology research [J Parasitol Res] 2017; Vol. 2017, pp. 2583969. Date of Electronic Publication: 2017 Feb 28.
DOI: 10.1155/2017/2583969
Abstrakt: Schistosomiasis is a Neglected Tropical Diseases which can be prevented with mass deworming chemotherapy. The reliance on a single drug, praziquantel, is a motivation for the search of novel antischistosomal compounds. This study investigated the anthelmintic activity of the stem bark and roots of Rauwolfia vomitoria against two life stages of Schistosoma mansoni . Both plant parts were found to be active against cercariae and adult worms. Within 2 h of exposure all cercariae were killed at a concentration range of 62.5-1000  µ g/mL and 250-1000  µ g/mL of R. vomitoria stem bark and roots, respectively. The LC 50 values determined for the stem bark after 1 and 2 h of exposure were 207.4 and 61.18  µ g/mL, respectively. All adult worms exposed to the concentrations range of 250-1000  µ g/mL for both plant parts died within 120 h of incubation. The cytotoxic effects against HepG2 and Chang liver cell assessed using MTT assay method indicated that both plant extracts which were inhibitory to the proliferation of cell lines with IC 50 > 20  μ g/mL appear to be safe. This report provides the first evidence of in vitro schistosomicidal potency of R. vomitoria with the stem bark being moderately, but relatively, more active and selective against schistosome parasites. This suggests the presence of promising medicinal constituent(s).
Competing Interests: The authors declare that they have no competing interests
Databáze: MEDLINE
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