| Autor: |
Zarzar LD; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.; Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139., Kalow JA; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.; Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139., He X; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.; Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139., Walish JJ; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.; Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139., Swager TM; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139; tswager@mit.edu.; Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139. |
| Abstrakt: |
In this paper, we describe an approach to measuring enzyme activity based on the reconfiguration of complex emulsions. Changes in the morphology of these complex emulsions, driven by enzyme-responsive surfactants, modulate the transmission of light through a sample. Through this method we demonstrate how simple photodetector measurements may be used to monitor enzyme kinetics. This approach is validated by quantitative measurements of enzyme activity for three different classes of enzymes (amylase, lipase, and sulfatase), relying on two distinct mechanisms for coupling droplet morphology to enzyme activity (host-guest interactions with uncaging and molecular cleavage). |
