3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells.

Autor: Mutsenko VV; Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya Str. 23, 61015 Kharkov, Ukraine; Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany. Electronic address: mutsenko@imp.uni-hannover.de., Bazhenov VV; Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger Straße 23, 09599 Freiberg, Germany., Rogulska O; Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya Str. 23, 61015 Kharkov, Ukraine., Tarusin DN; Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya Str. 23, 61015 Kharkov, Ukraine., Schütz K; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Brüggemeier S; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Gossla E; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Akkineni AR; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Meißner H; Department of Prosthetic Dentistry, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Lode A; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Meschke S; BromMarin GmbH, Wernerstraße 1, 09599 Freiberg, Germany., Ehrlich A; BromMarin GmbH, Wernerstraße 1, 09599 Freiberg, Germany., Petović S; Institute of Marine Biology, Institute of marine biology, University of Montenegro, 85330 Kotor, Montenegro., Martinović R; Institute of Marine Biology, Institute of marine biology, University of Montenegro, 85330 Kotor, Montenegro., Djurović M; Institute of Marine Biology, Institute of marine biology, University of Montenegro, 85330 Kotor, Montenegro., Stelling AL; Department of Biochemistry, Duke University School of Medicine, 27710 Durham, North Carolina, USA., Nikulin S; Moscow Institute of Physics and Technology, Institutskii Per. 9, 141700, Dolgoprudny, Moscow Region, Russia., Rodin S; P.A. Hertsen Moscow Research Oncology Institute, Botkinskii p. 3, 125284 Moscow, Russia., Tonevitsky A; P.A. Hertsen Moscow Research Oncology Institute, Botkinskii p. 3, 125284 Moscow, Russia., Gelinsky M; Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus of Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany., Petrenko AY; Institute for Problems of Cryobiology and Cryomedicine of the NAS Ukraine, Pereyaslavskaya Str. 23, 61015 Kharkov, Ukraine., Glasmacher B; Institute for Multiphase Processes, Leibniz Universität Hannover, Callinstraße 36, 30167 Hannover, Germany., Ehrlich H; Institute of Experimental Physics, TU Bergakademie Freiberg, Leipziger Straße 23, 09599 Freiberg, Germany. Electronic address: Hermann.Ehrlich@physik.tu-freiberg.de.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2017 Nov; Vol. 104 (Pt B), pp. 1966-1974. Date of Electronic Publication: 2017 Mar 25.
DOI: 10.1016/j.ijbiomac.2017.03.116
Abstrakt: The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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