| Autor: |
Grimes JA; 1 Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL, USA.; 2 Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA, USA., Matz BM; 1 Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL, USA., Christopherson PW; 3 Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA., Koehler JW; 3 Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, USA., Cappelle KK; 1 Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL, USA., Hlusko KC; 1 Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL, USA., Smith A; 1 Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, AL, USA. |
| Abstrakt: |
Melanocytic neoplasms are common in dogs and frequently occur within the oral cavity or in haired skin. The behavior of melanocytic neoplasms is variable and depends on tumor location, size, and histopathologic features. This study compared cytopathology and histopathology of 32 lymph nodes from 27 dogs diagnosed with melanocytic neoplasms. Agreement between the original cytology report, cytology slide review, original histopathology report, and histopathology slide review was determined for each lymph node. A subset of lymph nodes was subjected to immunohistochemistry (Melan-A) and additional histochemical stains/techniques (Prussian blue, bleach) to assist in differentiation of melanocytes and melanophages. Agreement ranged from slight to fair for each of the variables evaluated with weighted kappa (κ w ) or kappa (κ) analysis (original cytology vs cytology review κ w = 0.24; original cytology vs original histopathology κ w = 0.007; original cytology vs histopathology review κ w = 0.23; cytology review vs original histopathology κ w = 0.008; cytology review vs histopathology review κ w = 0.006; and original histopathology vs histopathology review κ = 0.18). The diagnoses (metastatic, equivocal, or negative for metastasis) of the original report and slide review for both cytology and histopathology were not significantly correlated with survival in this population of patients. Overall, agreement between cytology and histopathology was poor even with a single clinical or anatomic pathologist performing slide review. Consensus between routine cytology and histopathology for staging of lymph nodes in patients with melanocytic neoplasms is poor and does not correlate with survival. |
