Tubulointerstitial nephritis antigen-like 1 protein is downregulated in the placenta of pre-eclamptic women.

Autor: Mary S; Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra 411008 India., Kulkarni MJ; Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra 411008 India., Mehendale SS; Department of Gynecology, Bharati Vidyapeeth Medical College, Pune, Maharashtra 411043 India., Joshi SR; Department of Nutritional Medicine, Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune, Maharashtra 411043 India., Giri AP; Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra 411008 India.
Jazyk: angličtina
Zdroj: Clinical proteomics [Clin Proteomics] 2017 Mar 21; Vol. 14, pp. 8. Date of Electronic Publication: 2017 Mar 21 (Print Publication: 2017).
DOI: 10.1186/s12014-017-9144-2
Abstrakt: Background: Tubulointerstitial nephritis antigen-like 1 protein (TINAGL1), is a matricellular protein, known to play role in cell adhesion and cell receptor interaction. Research related to TINAGL1 is limited to cell culture and animal models. Demonstration of TINAGL1 as a positive regulator of angiogenesis and its expression in the decidua of postimplantation mouse uterus, prompted us to validate its expression in human placenta during impaired angiogenesis in pre-eclamptic condition.
Methods: Placental tissue from normotensive (n = 25) and pre-eclamptic (n = 25) pregnancies were used to study the differentially expressed proteins by two-dimensional gel electrophoresis and TINAGL1 protein was validated with Western blotting.
Results: A total of 55 protein spots were differentially expressed (fold change >1.5, p < 0.05), of which 27 were upregulated and 28 were downregulated in the pre-eclamptic placenta. TINAGL1 was found to be downregulated in pre-eclamptic compared to normotensive pregnant women.
Conclusion: This is the first study reporting TINAGL1 to be present in human placenta and differentially expressed in pre-eclamptic condition. The functional role of TINAGL1 in association to human pregnancy needs to be explored further.
Databáze: MEDLINE
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