Novel synthesis of gold nanoparticles using Artemisia vulgaris L. leaf extract and their efficacy of larvicidal activity against dengue fever vector Aedes aegypti L.

Autor: Sundararajan B; Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India., Ranjitha Kumari BD; Department of Plant Science, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India. Electronic address: ranjithakumari2004@yahoo.co.in.
Jazyk: angličtina
Zdroj: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2017 Sep; Vol. 43, pp. 187-196. Date of Electronic Publication: 2017 Mar 18.
DOI: 10.1016/j.jtemb.2017.03.008
Abstrakt: The Aedes aegypti L. mosquito transmits dengue and yellow fever, which cause millions of death every year. Dengue is a mosquito-borne viral disease that has rapidly spread worldwide particularly in countries with tropical and subtropical climates areas. The present study denotes a simple and eco-friendly biosynthesis of gold nanoparticles using Artemisia vulgaris L. leaf extract as reducing agent. The synthesized gold nanoparticles were characterized by UV-Visible Spectroscopy, X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Dynamic Light Scattering (DLS), Zeta Potential (ZP), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray Spectroscopy (EDX). Solid state 13 C NMR was utilized to confirm the presence of larvicidal compound Beta caryophyllene in the synthesized AuNPs. Larvicidal activity of the synthesized AuNPs was measured against A. aegypti over 12 and 24h exposure periods and compared with essential oil in various concentrations (25ppm, 50ppm, 100ppm, 200ppm and 400ppm). After a 12h exposure period, the larvicidal activity of 3 rd instar larva by AuNPs showed LC 50 =156.55ppm and LC 90 =2506.21ppm, while and essential oil displayed LC 50 =128.99ppm and LC 90 =1477.08ppm. Larvicidal activity of 4 th instar larva by AuNPs showed LC 50 =97.90ppm and LC 90 =1677.36ppm, while essential oil displayed LC 50 =136.15ppm and LC 90 =2223.55ppm. After a 24h of exposure period, larvicidal activity of 3 rd instar larva by AuNPs showed LC 50 =62.47ppm and LC 90 =430.16ppm and essential oil showed LC 50 =111.15ppm and LC 90 =1441.51ppm. The larvicidal activity of 4 th instar larva and AuNPs displayed LC 50 =43.01ppm and LC 90 =376.70ppm and for essential oil LC 50 =74.42ppm, LC 90 =858.36ppm. Histopathology of A. aegypti with AuNPs for 3 rd and 4 th stage larvae after 24h exposure at the highest mortality concentration (400ppm) showed that the area of the midgut, epithelial cells and cortex were highly affected. The present findings demonstrate that the biosynthesis of AuNPs using A. vulgaris leaf extracts could be an eco-friendly, safer nanobiopesticide and treatment against A. aegypti which could be used to combat of dengue fever.
(Copyright © 2017. Published by Elsevier GmbH.)
Databáze: MEDLINE
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