Autor: |
Nunes CF; Instituto de Ciências Agrárias, Universidade Federal de Minas Gerais, Montes Claros, MG, Brasil claudineia.f.nunes@gmail.com., Setotaw TA; Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil., Pasqual M; Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil., Chagas EA; Centro de Pesquisa Agroflorestal de Roraima, Empresa Brasileira de Pesquisa Agropecuária, Boa Vista, RR, Brasil., Santos EG; Laboratório de Biotecnologia Vegetal, Empresa de Pesquisa Agropecuária de Minas Gerais, Caldas, MG, Brasil., Santos DN; Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil., Lima CG; Centro de Pesquisa Agroflorestal de Roraima, Empresa Brasileira de Pesquisa Agropecuária, Boa Vista, RR, Brasil., Cançado GM; Unidade Mista de Pesquisa em Genômica Aplicada a Mudanças Climáticas, Empresa Brasileira de Pesquisa Agropecuária, Campinas, SP, Brasil. |
Abstrakt: |
Myrciaria dubia (camu-camu) is an Amazon tree that produces a tart fruit with high vitamin C content. It is probably the fruit with the highest vitamin C content among all Brazilian fruit crops and it can be used to supplement daily vitamin C dose. This property has attracted the attention of consumers and, consequently, encouraged fruit farmers to produce it. In order to identify and select potential accessions for commercial exploitation and breeding programs, M. dubia has received considerable research attention. The identification and characterization of genetic diversity, as well as identification of the population structure of accessions preserved in germplasm banks are fundamental for the success of any breeding program. The objective of this study was to evaluate the genetic variability of 10 M. dubia populations obtained from the shores of Reis Lake, located in the municipality of Caracaraí, Roraima, Brazil. Fourteen polymorphic inter simple sequence repeat (ISSR) markers were used to study the population genetic diversity, which resulted in 108 identified alleles. Among the 14 primers, GCV, UBC810, and UBC827 produced the highest number of alleles. The study illustrated the suitability and efficiency of ISSR markers to study the genetic diversity of M. dubia accessions. We also revealed the existence of high genetic variability among both accessions and populations that can be exploited in future breeding programs and conservation activities of this species. |