| Autor: |
Bernhard Y; Institut de Chimie Moléculaire, ICMUB CNRS UMR6302, University of Burgundy Franche-Comté, 9 avenue Alain Savary, 21078, Dijon, France., Collin B; Institut de Chimie Moléculaire, ICMUB CNRS UMR6302, University of Burgundy Franche-Comté, 9 avenue Alain Savary, 21078, Dijon, France.; Centre George-François Leclerc (CGFL), 1 rue du Professeur Marion, 21079, Dijon, France., Decréau RA; Institut de Chimie Moléculaire, ICMUB CNRS UMR6302, University of Burgundy Franche-Comté, 9 avenue Alain Savary, 21078, Dijon, France. |
| Abstrakt: |
Cherenkov Radiation (CR), this blue glow seen in nuclear reactors, is an optical light originating from energetic β-emitter radionuclides. CR emitter 90 Y triggers a cascade of energy transfers in the presence of a mixed population of fluorophores (which each other match their respective absorption and emission maxima): Cherenkov Radiation Energy Transfer (CRET) first, followed by multiple Förster Resonance Energy transfers (FRET): CRET ratios were calculated to give a rough estimate of the transfer efficiency. While CR is blue-weighted (300-500 nm), such cascades of Energy Transfers allowed to get a) fluorescence emission up to 710 nm, which is beyond the main CR window and within the near-infrared (NIR) window where biological tissues are most transparent, b) to amplify this emission and boost the radiance on that window: EMT6-tumor bearing mice injected with both a radionuclide and a mixture of fluorophores having a good spectral overlap, were shown to have nearly a two-fold radiance boost (measured on a NIR window centered on the emission wavelength of the last fluorophore in the Energy Transfer cascade) compared to a tumor injected with the radionuclide only. Some CR embarked light source could be converted into a near-infrared radiation, where biological tissues are most transparent. |
