Measurements of translation initiation from all 64 codons in E. coli.

Autor:	Hecht A; Joint Initiative for Metrology in Biology, Stanford, CA 94305, USA.; Genome-scale Measurements Group, National Institute of Standards and Technology, Stanford, CA 94305, USA.; Department of Bioengineering, Stanford, CA 94305, USA., Glasgow J; Joint Initiative for Metrology in Biology, Stanford, CA 94305, USA.; Genome-scale Measurements Group, National Institute of Standards and Technology, Stanford, CA 94305, USA.; Department of Bioengineering, Stanford, CA 94305, USA., Jaschke PR; Department of Bioengineering, Stanford, CA 94305, USA.; Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia., Bawazer LA; Joint Initiative for Metrology in Biology, Stanford, CA 94305, USA.; Genome-scale Measurements Group, National Institute of Standards and Technology, Stanford, CA 94305, USA.; Department of Bioengineering, Stanford, CA 94305, USA., Munson MS; Joint Initiative for Metrology in Biology, Stanford, CA 94305, USA.; Genome-scale Measurements Group, National Institute of Standards and Technology, Stanford, CA 94305, USA.; Department of Bioengineering, Stanford, CA 94305, USA., Cochran JR; Joint Initiative for Metrology in Biology, Stanford, CA 94305, USA.; Department of Bioengineering, Stanford, CA 94305, USA., Endy D; Joint Initiative for Metrology in Biology, Stanford, CA 94305, USA.; Department of Bioengineering, Stanford, CA 94305, USA., Salit M; Joint Initiative for Metrology in Biology, Stanford, CA 94305, USA.; Genome-scale Measurements Group, National Institute of Standards and Technology, Stanford, CA 94305, USA.; Department of Bioengineering, Stanford, CA 94305, USA.
Jazyk:	angličtina
Zdroj:	Nucleic acids research [Nucleic Acids Res] 2017 Apr 20; Vol. 45 (7), pp. 3615-3626.
DOI:	10.1093/nar/gkx070
Abstrakt:	Our understanding of translation underpins our capacity to engineer living systems. The canonical start codon (AUG) and a few near-cognates (GUG, UUG) are considered as the 'start codons' for translation initiation in Escherichia coli. Translation is typically not thought to initiate from the 61 remaining codons. Here, we quantified translation initiation of green fluorescent protein and nanoluciferase in E. coli from all 64 triplet codons and across a range of DNA copy number. We detected initiation of protein synthesis above measurement background for 47 codons. Translation from non-canonical start codons ranged from 0.007 to 3% relative to translation from AUG. Translation from 17 non-AUG codons exceeded the highest reported rates of non-cognate codon recognition. Translation initiation from non-canonical start codons may contribute to the synthesis of peptides in both natural and synthetic biological systems. (Published by Oxford University Press on behalf of Nucleic Acids Research 2017.)
Databáze:	MEDLINE
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