| Autor: |
Alessio GD; Laboratório de Doença de Chagas, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Instituto de Ciências Exatas e Biológicas (ICEB), Universidade Federal de Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., de Araújo FF; Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil., Côrtes DF; Laboratório de Doença de Chagas, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Instituto de Ciências Exatas e Biológicas (ICEB), Universidade Federal de Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil., Sales Júnior PA; Grupo de Genômica Funcional e Proteômica de Leishmania spp e Trypanosoma cruzi, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil., Lima DC; Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil., Gomes MS; Laboratório de Bioinformática e Análises Moleculares, Universidade Federal de Uberlândia, INGEB/FACOM, Campus Patos de Minas, Patos de Minas, Minas Gerais, Brazil., do Amaral LR; Laboratório de Bioinformática e Análises Moleculares, Universidade Federal de Uberlândia, INGEB/FACOM, Campus Patos de Minas, Patos de Minas, Minas Gerais, Brazil., Xavier MA; Grupo de Pesquisas Clínicas e Políticas Públicas em Doenças Infecciosas e Parasitárias, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil., Teixeira-Carvalho A; Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil., Martins-Filho OA; Grupo Integrado de Pesquisas em Biomarcadores, Centro de Pesquisas René Rachou (CPqRR-FIOCRUZ/ MG), Belo Horizonte, Minas Gerais, Brazil., de Lana M; Laboratório de Doença de Chagas, Núcleo de Pesquisas em Ciências Biológicas (NUPEB), Instituto de Ciências Exatas e Biológicas (ICEB), Universidade Federal de Ouro Preto (UFOP), Ouro Preto, Minas Gerais, Brazil. |
| Abstrakt: |
Distinct Trypanosoma cruzi genotypes have been considered relevant for patient management and therapeutic response of Chagas disease. However, typing strategies for genotype-specific serodiagnosis of Chagas disease are still unavailable and requires standardization for practical application. In this study, an innovative TcI/TcVI/TcII Chagas Flow ATE-IgG2a technique was developed with applicability for universal and genotype-specific diagnosis of T. cruzi infection. For this purpose, the reactivity of serum samples (percentage of positive fluorescent parasites-PPFP) obtained from mice chronically infected with TcI/Colombiana, TcVI/CL or TcII/Y strain as well as non-infected controls were determined using amastigote-AMA, trypomastigote-TRYPO and epimastigote-EPI in parallel batches of TcI, TcVI and TcII target antigens. Data demonstrated that "α-TcII-TRYPO/1:500, cut-off/PPFP = 20%" presented an excellent performance for universal diagnosis of T. cruzi infection (AUC = 1.0, Se and Sp = 100%). The combined set of attributes "α-TcI-TRYPO/1:4,000, cut-off/PPFP = 50%", "α-TcII-AMA/1:1,000, cut-off/PPFP = 40%" and "α-TcVI-EPI/1:1,000, cut-off/PPFP = 45%" showed good performance to segregate infections with TcI/Colombiana, TcVI/CL or TcII/Y strain. Overall, hosts infected with TcI/Colombiana and TcII/Y strains displayed opposite patterns of reactivity with "α-TcI TRYPO" and "α-TcII AMA". Hosts infected with TcVI/CL strain showed a typical interweaved distribution pattern. The method presented a good performance for genotype-specific diagnosis, with global accuracy of 69% when the population/prototype scenario include TcI, TcVI and TcII infections and 94% when comprise only TcI and TcII infections. This study also proposes a receiver operating reactivity panel, providing a feasible tool to classify serum samples from hosts infected with distinct T. cruzi genotypes, supporting the potential of this method for universal and genotype-specific diagnosis of T. cruzi infection. |
