Real-time in vivo monitoring of magnetic nanoparticles in the bloodstream by AC biosusceptometry.

Autor:	Próspero AG; Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil., Quini CC; Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil., Bakuzis AF; Physics Institute, Federal University of Goiás, Goiânia, Goiás, Brazil. abakuzis@ufg.br.; Instituto de Física-Universidade Federal de Goiás, Goiânia, GO, 74690-900, Brazil. abakuzis@ufg.br., Fidelis-de-Oliveira P; Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil., Moretto GM; Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil., Mello FP; Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil., Calabresi MF; Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil., Matos RV; Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil., Zandoná EA; Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil., Zufelato N; Physics Institute, Federal University of Goiás, Goiânia, Goiás, Brazil., Oliveira RB; Ribeirão Preto School of Medicine, São Paulo University, Ribeirão Prêto, São Paulo, Brazil., Miranda JR; Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of nanobiotechnology [J Nanobiotechnology] 2017 Mar 21; Vol. 15 (1), pp. 22. Date of Electronic Publication: 2017 Mar 21.
DOI:	10.1186/s12951-017-0257-6
Abstrakt:	Background: We introduce and demonstrate that the AC biosusceptometry (ACB) technique enables real-time monitoring of magnetic nanoparticles (MNPs) in the bloodstream. We present an ACB system as a simple, portable, versatile, non-invasive, and accessible tool to study pharmacokinetic parameters of MNPs, such as circulation time, in real time. We synthesized and monitored manganese doped iron oxide nanoparticles in the bloodstream of Wistar rats using two different injection protocols. Aiming towards a translational approach, we also simultaneously evaluated cardiovascular parameters, including mean arterial pressure, heart rate, and episodes of arrhythmia in order to secure the well-being of all animals. Results: We found that serial injections increased the circulation time compared with single injections. Immediately after each injection, we observed a transitory drop in arterial pressure, a small drop in heart rate, and no episodes of arrhythmia. Although some cardiovascular effects were observed, they were transitory and easily recovered in both protocols. Conclusions: These results indicate that the ACB system may be a valuable tool for in vivo, real-time MNP monitoring that allows associations with other techniques, such as pulsatile arterial pressure and electrocardiogram recordings, helping ensuring the protocol safety, which is a fundamental step towards clinical applications.
Databáze:	MEDLINE
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