| Autor: |
Peitsch MC; Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland., Kovacsovics TJ, Isliker H |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunological methods [J Immunol Methods] 1988 Apr 06; Vol. 108 (1-2), pp. 265-9. |
| DOI: |
10.1016/0022-1759(88)90428-0 |
| Abstrakt: |
The purification of the subcomponents C1r and C1s of the first component of complement involves multiple steps and is time-consuming. This accounts for the frequently observed partial activation of the subcomponents. In this report we propose a simplified procedure of purification using a batch method and fast protein chromatography avoiding a shift of pH. The method provides C1r and C1s in a yield of 35 and 60% respectively. In addition, this study provides a simple and sensitive test to assess functional purity of C1r and C1s with respect to the other C1 subcomponents. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
