Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition.

Autor:	Huang Y; Wellcome Trust/Cancer Research United Kingdom Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom., Kim JK; European Molecular Biology Laboratory, European Bioinformatics Institute, Cambridge, United Kingdom.; Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology, Daegu, Republic of Korea., Do DV; Wellcome Trust/Cancer Research United Kingdom Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom., Lee C; Wellcome Trust/Cancer Research United Kingdom Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom., Penfold CA; Wellcome Trust/Cancer Research United Kingdom Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom., Zylicz JJ; Wellcome Trust/Cancer Research United Kingdom Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom., Marioni JC; European Molecular Biology Laboratory, European Bioinformatics Institute, Cambridge, United Kingdom.; Wellcome Trust Sanger Institute, Cambridge, United Kingdom.; Cancer Research United Kingdom Cambridge Institute, University of Cambridge, Cambridge, United Kingdom., Hackett JA; Wellcome Trust/Cancer Research United Kingdom Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom.; European Molecular Biology Laboratory - Monterotondo, Rome, Italy., Surani MA; Wellcome Trust/Cancer Research United Kingdom Gurdon Institute, University of Cambridge, Cambridge, United Kingdom.; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2017 Mar 21; Vol. 6. Date of Electronic Publication: 2017 Mar 21.
DOI:	10.7554/eLife.22345
Abstrakt:	The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by Dppa3 ) using single-cell/embryo approaches. We show that loss of maternal Stella results in widespread transcriptional mis-regulation and a partial failure of MZT. Strikingly, activation of endogenous retroviruses (ERVs) is significantly impaired in Stella maternal/zygotic knockout embryos, which in turn leads to a failure to upregulate chimeric transcripts. Amongst ERVs, MuERV-L activation is particularly affected by the absence of Stella, and direct in vivo knockdown of MuERV-L impacts the developmental potential of the embryo. We propose that Stella is involved in ensuring activation of ERVs, which themselves play a potentially key role during early development, either directly or through influencing embryonic gene expression.
Databáze:	MEDLINE
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