Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials.

Autor: Szczepankowska AK; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland., Szatraj K; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland., Sałański P; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland., Rózga A; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland., Górecki RK; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland., Bardowski JK; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2017; Vol. 2017, pp. 6747482. Date of Electronic Publication: 2017 Feb 22.
DOI: 10.1155/2017/6747482
Abstrakt: Lactic acid bacteria (LAB) are Gram-positive, nonpathogenic microorganisms that are gaining much interest as antigen producers for development of live vaccine vectors. Heterologous proteins of different origin have been successfully expressed in various LAB species, including Lactococcus lactis . Recombinant L. lactis strains have been shown to induce specific local and systemic immune responses against various antigens. Our study aimed at constructing a L. lactis strain expressing haemagglutinin of a Polish avian H5H1 influenza isolate and examining its effect on animals. Expression of the cloned H5 gene was achieved using the nisin-controlled gene expression system. Detection of the intracellular H5 antigen produced in L. lactis was performed by Western blot analysis and confirmed using mass spectrometry. The potential of L. lactis recombinant cells to induce an immune response was examined by setting up preliminary immunization trials on chickens and mice. Obtained sera were tested for specific antibodies by ELISA assays. The results of these studies are a promising step toward developing a vaccine against the bird flu using Lactococcus lactis cells as bioreactors for efficient antigen production and delivery to the mucosal surface.
Competing Interests: The authors declare that there are no competing interests regarding the publication of this paper.
Databáze: MEDLINE
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