| Autor: |
Viktorov AV, Dank EKh, Kuznetsov VA, Ter-Simonian VG, Iurkiv VA |
| Jazyk: |
ruština |
| Zdroj: |
Biulleten' eksperimental'noi biologii i meditsiny [Biull Eksp Biol Med] 1988 Mar; Vol. 105 (3), pp. 291-4. |
| Abstrakt: |
The interaction of lipopolysaccharide toxin (LPS) with isolated washed human blood platelets has been studied. LPS was found to induce the rapid (1-2 min) and marked (15-20%) breakdown of mono- and polyphosphoinositides and formation of significant amounts of diacylglycerols (ca. 20%). However TxB2 biosynthesis from endogenous 14C-arachidonic acid was stimulated by LPS incubation only by ca. 20%. Phosphatidylcholine and phosphatidylethanolamine were also hydrolysed by ca. 8 and 12%, respectively, presumably via the activation of endogenous phospholipase A2. Besides, LPS caused the decreasing of the lipid fluidity of a platelet plasma membrane as was shown by ESR spectroscopy using doxylstearic acid probes. All these changes by LPS induce no aggregation of platelets. It is concluded that an enhancement of a phosphoinositide cycle is not a possibly necessary and sufficient condition for a platelet aggregation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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