| Autor: |
Homayouni MM; Department of Parasitology and Mycology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran., Rostami A; Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Gholizadeh H; Department of Parasitology and Mycology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran., Mehbod ASA; Department of Parasitology and Mycology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran., Ebrahimi M; Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Mehravar S; Department of Epidemiology and Statistics, School of Public Health, Tehran University of Medical Science, Tehran, Iran. |
| Abstrakt: |
Pneumocystis pneumonia due to Pneumocystis jirovecii infection is an emerging health problem not only for HIV-infected patients but also for other immunocompromised patients in many countries. We compared Gomori methenamine silver (GMS), Toluidine Blue O (TBO) and Giemsa staining methods using standard procedures. The sensitivity and specificity of GMS were 100 %. The sensitivity and specificity of TBO were 96 and 100 %, respectively. The sensitivity and specificity of Giemsa stain were 84 and 90 %, respectively. Only GMS had positive and negative predictive values of 100 % while PPV and NPV for TBO were 100 and 90.9 %, and for Giemsa stain were 95.4 and 69.2 %, respectively. Therefore, our results suggest that if TBO or Geimsa stains are used as the primary staining methods in a clinical laboratory, then confirmation with a GMS staining method should be performed to increase the sensitivity and specificity of the final test result. |
Full text from SpringerLink