In vitro effects of phthalate esters in human myometrial and leiomyoma cells and increased urinary level of phthalate metabolite in women with uterine leiomyoma.

Autor:	Kim JH; Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea., Kim SH; Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea. Electronic address: kimsung@amc.seoul.kr., Oh YS; Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea., Ihm HJ; Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea., Chae HD; Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea., Kim CH; Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea., Kang BM; Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea.
Jazyk:	angličtina
Zdroj:	Fertility and sterility [Fertil Steril] 2017 Apr; Vol. 107 (4), pp. 1061-1069.e1. Date of Electronic Publication: 2017 Mar 11.
DOI:	10.1016/j.fertnstert.2017.01.015
Abstrakt:	Objective: To investigate the possible role of phthalate, a ubiquitous chemical used in consumer products, in the pathogenesis of uterine leiomyoma. Design: Experimental and prospective case-control study using human samples. Setting: University hospital. Patient(s): Fifty-three women with histologic evidence of uterine leiomyoma and 33 surgical controls without leiomyoma. Intervention(s): Human myometrial and leiomyoma cells were treated with di-(2-thylhexyl)-phthalate (DEHP). Main Outcome Measure(s): Cell viability assay and Western blot analyses after in vitro DEHP treatment; high-performance liquid chromatography electrospray ionization tandem mass spectrometry in cases and controls. Result(s): In vitro treatment with DEHP led to an increased viability and increased expressions of proliferating cell nuclear antigen, B-cell lymphoma 2 protein, and type I collagen in myometrial and leiomyoma cells. The urinary concentration of mono-(2-ethyl-5-carboxypentyl) phthalate was higher in women with leiomyoma compared with controls. Conclusion(s): These findings suggest that exposure to phthalate may play a role in the pathogenesis of uterine leiomyoma by enhancing proliferative activity, exerting an antiapoptotic effect, and increasing collagen contents in myometrial and leiomyoma cells. (Copyright © 2017 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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