MicroRNA expression profiles in human CD3 + T cells following stimulation with anti-human CD3 antibodies.
| Autor: | Sousa IG; Molecular Pathology Graduation Program, Medicine Faculty, University of Brasilia, Brasilia, Brazil., do Almo MM; Molecular Pathology Graduation Program, Medicine Faculty, University of Brasilia, Brasilia, Brazil., Simi KC; Molecular Biology Graduation Program, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil., Bezerra MA; Molecular Biology Graduation Program, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil., Andrade RV; Catholic University of Brasília, Brasilia, Brazil., Maranhão AQ; Department of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil.; Institute for Immunology Investigation, A National Institute of Science and Technology, Brasilia, Brazil., Brigido MM; Department of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia, Brazil. brigido@unb.br.; Institute for Immunology Investigation, A National Institute of Science and Technology, Brasilia, Brazil. brigido@unb.br. |
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| Jazyk: | angličtina |
| Zdroj: | BMC research notes [BMC Res Notes] 2017 Mar 14; Vol. 10 (1), pp. 124. Date of Electronic Publication: 2017 Mar 14. |
| DOI: | 10.1186/s13104-017-2442-y |
| Abstrakt: | Background: Anti-CD3 therapy can induce immunosuppression by several non mutually exclusive mechanisms that have been proposed to explain the therapeutic effect the administration anti-CD3 mAb, but its immunoregulatory mechanism is still not completely clear. In T cells, microRNAs (miRNAs) regulate several pathways, including those associated with immune tolerance. Here, we report changes in miRNA expression in T cells following treatment with anti-human CD3 antibodies. Peripheral blood mononuclear cells were cultured in the presence of the monoclonal antibody OKT3 or a recombinant fragment of humanized anti-CD3. Following these treatments, the expression profiles of 31 miRNA species were assessed in T cells using TaqMan arrays. Results: Eight of the tested miRNAs (miR-155, miR-21, miR-146a, miR-210, miR-17, miR-590-5p, miR-106b and miR-301a) were statistically significantly up- or down-regulated relative to untreated cells. Conclusions: Stimulation of T cells with anti-human CD3 antibodies alters miRNA expression patterns, including of miRNA species associated with immune regulatory pathways. |
| Databáze: | MEDLINE |
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