Identification of compounds with cytotoxic activity from the leaf of the Nigerian medicinal plant, Anacardium occidentale L. (Anacardiaceae).

Autor: Taiwo BJ; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife, Nigeria; School of Pharmacy, Faculty of Life Sciences, University of Bradford, Bradford BD7 1DP, England, United Kingdom. Electronic address: bamit@oauife.edu.ng., Fatokun AA; School of Pharmacy, Faculty of Life Sciences, University of Bradford, Bradford BD7 1DP, England, United Kingdom. Electronic address: A.A.Fatokun@ljmu.ac.uk., Olubiyi OO; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Obafemi Awolowo University, Ile Ife, Nigeria., Bamigboye-Taiwo OT; Department of Physiology, Faculty of Basic Medical Science, Obafemi Awolowo University, Ile Ife, Nigeria; Department of Paediatrics, Federal Teaching Hospital, Ido-Ekiti, Ekiti State, Nigeria., van Heerden FR; School of Chemistry and Physics, Pietermaritzburg Campus, University of KwaZulu-Natal, Private Bag X01, Scottsville 3209, South Africa., Wright CW; School of Pharmacy, Faculty of Life Sciences, University of Bradford, Bradford BD7 1DP, England, United Kingdom.
Jazyk: angličtina
Zdroj: Bioorganic & medicinal chemistry [Bioorg Med Chem] 2017 Apr 15; Vol. 25 (8), pp. 2327-2335. Date of Electronic Publication: 2017 Feb 22.
DOI: 10.1016/j.bmc.2017.02.040
Abstrakt: Cancer is now the second-leading cause of mortality and morbidity, behind only heart disease, necessitating urgent development of (chemo)therapeutic interventions to stem the growing burden of cancer cases and cancer death. Plants represent a credible source of promising drug leads in this regard, with a long history of proven use in the indigenous treatment of cancer. This study therefore investigated Anacardium occidentale, one of the plants in a Nigerian Traditional Medicine formulation commonly used to manage cancerous diseases, for cytotoxic activity. Bioassay-guided fractionation, spectroscopy, Alamar blue fluorescence-based viability assay in cultured HeLa cells and microscopy were used. Four compounds, zoapatanolide A (1), agathisflavone (2), 1,2-bis(2,6-dimethoxy-4-methoxycarbonylphenyl)ethane (anacardicin, 3) and methyl gallate (4), were isolated, with the most potent being zoapatanolide A with an IC 50 value of 36.2±9.8µM in the viability assay. To gain an insight into the likely molecular basis of their observed cytotoxic effects, Autodock Vina binding free energies of each of the isolated compounds with seven molecular targets implicated in cancer development (MAPK8, MAPK10, MAP3K12, MAPK3, MAPK1, MAPK7 and VEGF), were calculated. Pearson correlation coefficients were obtained with experimentally-determined IC 50 in the Alamar blue viability assay. While these compounds were not as potent as a standard anticancer compound, doxorubicin, the results provide reasonable evidence that the plant species contains compounds with cytotoxic activity. This study provides some evidence of why this plant is used ethnobotanically in anticancer herbal formulations and justifies investigating Nigerian medicinal plants highlighted in recent ethnobotanical surveys.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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