Molecular detection of Hepatozoon spp. in domestic dogs and wild mammals in southern Pantanal, Brazil with implications in the transmission route.

Autor: de Sousa KC; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil., Fernandes MP; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil., Herrera HM; Universidade Católica Dom Bosco, Campo Grande, MS, Brazil., Benevenute JL; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil., Santos FM; Universidade Católica Dom Bosco, Campo Grande, MS, Brazil., Rocha FL; Universidade Federal da Paraíba, Laboratório de Ecologia Animal, Rio Tinto, PB, Brazil., Barreto WT; Universidade Católica Dom Bosco, Campo Grande, MS, Brazil., Macedo GC; Universidade Católica Dom Bosco, Campo Grande, MS, Brazil., Campos JB; Universidade Católica Dom Bosco, Campo Grande, MS, Brazil., Martins TF; Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP, Brazil., de Andrade Pinto PC; Universidade Federal da Paraíba, Laboratório de Ecologia Animal, Rio Tinto, PB, Brazil., Battesti DB; Laboratório de Parasitologia, Instituto Butantan, São Paulo, SP, Brazil., Piranda EM; Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil., Cançado PH; Embrapa gado de corte, Campo Grande, MS, Brazil., Machado RZ; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil., André MR; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista, UNESP, Jaboticabal, SP, Brazil. Electronic address: mandre.fcav@gmail.com.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2017 Apr 15; Vol. 237, pp. 37-46. Date of Electronic Publication: 2017 Feb 22.
DOI: 10.1016/j.vetpar.2017.02.023
Abstrakt: Hepatozoon parasites comprise intracellular apicomplexan parasites transmitted to vertebrate animals by ingestion of arthropods definitive hosts. The present work aimed to investigate the occurrence of Hepatozoon spp. in wild animals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil, by molecular techniques. Between August 2013 and March 2015, 31 coatis (Nasua nasua), 78 crab-eating foxes (Cerdocyon thous), seven ocelots (Leopardus pardalis), 42 dogs (Canis lupus familiaris), 110 wild rodents (77 Thichomys fosteri, 25 Oecomys mamorae, and 8 Clyomys laticeps), 30 marsupials (14 Thylamys macrurus, 11 Gracilinanus agilis, 4 Monodelphis domestica and 1 Didelphis albiventris), and 1582 ticks and 80 fleas collected from the sampled animals were investigated. DNA samples were submitted to PCR assays for Hepatozoon spp. targeting 18S rRNA gene. Purified amplicons were directly sequenced and submitted to phylogenetic analysis. A high prevalence of Hepatozoon among carnivores (C. thous [91.02%], dogs [45.23%], N. nasua [41.9%] and L. pardalis [71.4%]) was found. However, ticks and fleas were negative to Hepatozoon PCR assays. By phylogenetic analysis based on 18S rRNA sequences, Hepatozoon sequences amplified from crab-eating foxes, dogs, coatis and ocelots clustered with sequences of H. canis, H. americanum and H. felis. The closely related positioning of Hepatozoon sequences amplified from wild rodents and T. macrurus marsupial to Hepatozoon from reptiles and amphibians suggest a possible transmission of those Hepatozoon species between hosts by ectoparasites or by predation. Hepatozoon haplotypes found circulating in wild rodents seem to present a higher degree of polymorphism when compared to those found in other groups of animals. Although rodents seem not to participate as source of Hepatozoon infection to wild carnivores and domestic dogs, they may play an important role in the transmission of Hepatozoon to reptiles and amphibians in Pantanal biome.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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