[Detection of Autoantibodies Against the 1-Adrenergic Receptor in the Sera of Patients via the Competitive cell-Based Enzyme Linked Immunosorbent Assay].

Autor:	Shevelev AY; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Kostiukevich MV; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Efremov EE; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Vlasik TN; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Mironova NA; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Zykov KA; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Kashirina NM; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Kuznetsova IB; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Sharf TV; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Mamochkina EN; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Lipatova LN; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Peklo MM; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Rutkevich PN; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Yanushevskaya EV; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Rybalkin IN; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Stukalova OV; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Malkina TA; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Belyaeva MM; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Kuznetsova TV; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Tkachev GA; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Zinchenko LV; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Gupalo EM; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Agapova OY; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Yureneva-Tkhorzhevskaya TV; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Rvacheva AV; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Sidorova MV; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Sadgyan AS; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Tereshchenko SN; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia., Golitsyn SP; Russian Cardiology Scientific and Production Complex, Moscow, Russia.; Evdokimov Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, Russia.
Jazyk:	ruština
Zdroj:	Kardiologiia [Kardiologiia] 2016 Dec; Vol. 56 (11), pp. 61-70.
DOI:	10.18565/cardio.2016.11.61-70
Abstrakt:	Objective: This study aimed to assess the level of anti-1-adrenergic receptor autoantibodies in patients with ventricular arrhythmias with no signs of organic heart disease and with presence of cardiovascular pathology in comparison with a group of healthy volunteers. Material and Methods: The study included 44 patients with ventricular arrhythmias with no signs of organic heart disease ("idiopathic"), 34 patients with diagnosed dilated cardiomyopathy (DCM) of inflammatory origin, 35 patients with coronary heart disease and ventricular arrhythmias, 12patients with coronary heart disease with no ventricular arrhythmias, and 19 healthy volunteers (control group). The level of autoantibodies against the 1-adrenergic receptor was determined by the developed competitive cell-based enzyme-linked immunosorbent assay (ELISA) and by the standard ELISA using peptides corresponding to the second extracellular loop of the 1-adrenergic receptor. Results: Elevated level of autoantibodies detected by a competitive cell-based ELISA was observed in 62% of patients with DCM compared to 21% of healthy volunteers (p=0.0006). In patients with "idiopathic" ventricular arrhythmias, the level of 1-adrenergic receptor autoantibodies was lower than in healthy subjects (p=0.003). Coronary heart disease patients with or without ventricular arrhythmias exhibited no differences from the control group. The number of significantly positive signals in peptide-based ELISA did not exceed 10% in any of the groups. No correlation between the data from competitive cell-based ELISA and peptide-based ELISA was found. Conclusions: This study demonstrated that competitive cell-based ELISA technique can be applied for detection of 1-adrenergic receptor autoantibodies. The results in DCM patients generally correspond to the expected. Decreased level of autoantibodies in patients with "idiopathic" ventricular arrhythmias indicates that this disease is related to changes in the immune system. Such relation is not observed in the case of coronary heart disease patients.
Databáze:	MEDLINE
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