Worldwide circulation of HSV-2 × HSV-1 recombinant strains.

Autor: Koelle DM; Department of Medicine, University of Washington, Seattle, WA 98195, USA.; Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA.; Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.; Department of Global Health, University of Washington, Seattle, WA 98195, USA.; Benaroya Research Institute, Seattle, WA 98102, USA., Norberg P; Department of Infectious Diseases, University of Gothenburg, Guldhedsgatan 10B, 41346, Gothenburg, Sweden., Fitzgibbon MP; Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA., Russell RM; Department of Medicine, University of Washington, Seattle, WA 98195, USA., Greninger AL; Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA., Huang ML; Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA., Stensland L; Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA., Jing L; Department of Medicine, University of Washington, Seattle, WA 98195, USA., Magaret AS; Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA.; Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.; Department of Biostatistics, University of Washington, Seattle, WA 98195, USA., Diem K; Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA., Selke S; Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA., Xie H; Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA., Celum C; Department of Medicine, University of Washington, Seattle, WA 98195, USA.; Department of Global Health, University of Washington, Seattle, WA 98195, USA.; Department of Epidemiology, University of Washington, Seattle, WA 98195, USA., Lingappa JR; Department of Medicine, University of Washington, Seattle, WA 98195, USA.; Department of Global Health, University of Washington, Seattle, WA 98195, USA.; Department of Pediatrics, University of Washington, Seattle, WA 98195, USA., Jerome KR; Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA.; Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA., Wald A; Department of Medicine, University of Washington, Seattle, WA 98195, USA.; Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA.; Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.; Department of Epidemiology, University of Washington, Seattle, WA 98195, USA., Johnston C; Department of Medicine, University of Washington, Seattle, WA 98195, USA.; Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2017 Mar 13; Vol. 7, pp. 44084. Date of Electronic Publication: 2017 Mar 13.
DOI: 10.1038/srep44084
Abstrakt: Homo sapiens harbor two distinct, medically significant species of simplexviruses, herpes simplex virus (HSV)-1 and HSV-2, with estimated divergence 6-8 million years ago (MYA). Unexpectedly, we found that circulating HSV-2 strains can contain HSV-1 DNA segments in three distinct genes. Using over 150 genital swabs from North and South America and Africa, we detected recombinants worldwide. Common, widely distributed gene UL39 genotypes are parsimoniously explained by an initial >457 basepair (bp) HSV-1 × HSV-2 crossover followed by back-recombination to HSV-2. Blocks of >244 and >539 bp of HSV-1 DNA within genes UL29 and UL30, respectively, have reached near fixation, with a minority of strains retaining sequences we posit as ancestral HSV-2. Our data add to previous in vitro and animal work, implying that in vivo cellular co-infection with HSV-1 and HSV-2 yields viable interspecies recombinants in the natural human host.
Databáze: MEDLINE