Hypoxia reduces the E-cadherin expression and increases OSCC cell migration regardless of the E-cadherin methylation profile.

Autor: Domingos PLB; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., Souza MG; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., Guimarães TA; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., Santos ES; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., Farias LC; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., de Carvalho Fraga CA; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., Jones KM; Dean of Research, Associação Educativa do Brasil (SOEBRAS), Montes Claros, Montes Claros, Minas Gerais, Brazil., Santos SHS; Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Brazil., de Paula AMB; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., Guimarães ALS; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil. Electronic address: andreluizguimaraes@gmail.com.
Jazyk: angličtina
Zdroj: Pathology, research and practice [Pathol Res Pract] 2017 May; Vol. 213 (5), pp. 496-501. Date of Electronic Publication: 2017 Feb 20.
DOI: 10.1016/j.prp.2017.02.003
Abstrakt: Objective: The purpose of the current study is to investigate the association between E-cadherin methylation status, hypoxia and OSCC.
Methods: HaCat and SCC9 cell lines were submitted to hypoxic treatment, followed by methylation profile analysis (MS-PCR) and analysis of the expression of mRNA gene E-cadherin (RT-PCR). Study group samples comprise individuals affected by potentially malignant lesions Potential Malignant Oral Lesion (PMOL, n=18) and oral squamous cell carcinoma (OSCC, n=28). The control group oral mucosa (OM, n=15) of patients with an oral mucocele. Cell migration ability was evaluated a scratch wound assay in SCC9 and HaCat cell lines RESULTS: E-cadherin mRNA expression in the cell lines SCC9 and HaCat was significantly reduced under hypoxia, regardless of the methylation profile, when compared to the control group. No differences in methylation profile of the E-cadherin were observed among the groups OM, PMOL and OSCC. HaCat and SCC9 presented increases in cell migration rates under hypoxia.
Conclusion: The current study demonstrates that hypoxia reduces E-cadherin expression and increase cell migration, regardless of the methylation profile. Additionally, no differences in E-cadherin methylation patterns were observed among OM, PMOL and OSCC.
(Copyright © 2017 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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