Probiotic consumption decreases the number of osteoclasts during orthodontic movement in mice.

Autor: Pazzini CA; Department of Pediatric Dentistry and Orthodontics, Dental School, Universidade Federal de Minas Gerais - UFMG, Belo Horizonte, MG, Brazil. Electronic address: camilapazzini@hotmail.com., Pereira LJ; Department of Health Sciences, Universidade Federal de Lavras - UFLA, Lavras, MG, Brazil., da Silva TA; Department of Oral Surgery and Oral Pathology, Dental School, Universidade Federal de Minas Gerais - UFMG, Belo Horizonte, MG, Brazil., Montalvany-Antonucci CC; Department of Oral Surgery and Oral Pathology, Dental School, Universidade Federal de Minas Gerais - UFMG, Belo Horizonte, MG, Brazil., Macari S; Department of Oral Surgery and Oral Pathology, Dental School, Universidade Federal de Minas Gerais - UFMG, Belo Horizonte, MG, Brazil., Marques LS; Department of Pediatric Dentistry and Orthodontics, Dental School, Universidade Federal do Vale do Jequitinhonha e Mucuri - UFVJM, Diamantina, MG, Brazil., de Paiva SM; Department of Pediatric Dentistry and Orthodontics, Dental School, Universidade Federal de Minas Gerais - UFMG, Belo Horizonte, MG, Brazil.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2017 Jul; Vol. 79, pp. 30-34. Date of Electronic Publication: 2017 Feb 24.
DOI: 10.1016/j.archoralbio.2017.02.017
Abstrakt: Aims: The aim of the present study was to investigate the effect of probiotic (Bacillus Subtilis) supplementation on bone remodelling induced by mechanical loading.
Methods: C57BL/6 mice were divided in two groups: (1) Probiotic and (2) Vehicle (water). The probiotic (1.5×10 8 CFU/mL) was administered orally for 14 days, starting two days before the induction of orthodontic tooth movement (OTM). OTM was determined by histomorphometric analysis by comparing the right to the left side of the maxilla. The number of osteoclasts was determined by counting TRAP-positive cells. Osteoblasts were counted on Masson's trichrome-stained slides.
Results: OTM was similar between groups (with and without probiotic supplementation) (p=0.46). The number of TRAP-positive cells increased (p<0.01) on the experimental side (where the spring coil was installed) in comparison to the control side in both groups. However, the number of osteoclasts decreased (p˂0.01) in the probiotic group, in comparison to the vehicle group. There was an increase in the number of osteoblasts (p˂0.05) in both the Vehicle and Probiotic groups on the side under OTM, independent of probiotic supplementation.
Conclusion: Oral Supplementation with a probiotic influenced the number of osteoclasts adjacent to the tooth root during orthodontic movement in mice.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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