DNA-Directed Antibody Immobilization for Robust Protein Microarrays: Application to Single Particle Detection 'DNA-Directed Antibody Immobilization.

Autor:	Ünlü NL; Biomedical Engineering Department, Boston University, Boston, MA, 02215, USA.; Faculty of Medicine, Bahcesehir University, Istanbul, 34353, Turkey., Kanik FE; Electrical and Computer Engineering Department, Boston University, Boston, MA, 02215, USA., Seymour E; Biotechnology Research Program Department, ASELSAN Research Center, Ankara, 06370, Turkey., Connor JH; Microbiology Department, Boston University School of Medicine, 8 St Mary's St, Boston, MA, 02215, USA., Ünlü MS; Biomedical Engineering Department, Boston University, Boston, MA, 02215, USA. selim@bu.edu.; Electrical and Computer Engineering Department, Boston University, Boston, MA, 02215, USA. selim@bu.edu.; Microbiology Department, Boston University School of Medicine, 8 St Mary's St, Boston, MA, 02215, USA. selim@bu.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1571, pp. 187-206.
DOI:	10.1007/978-1-4939-6848-0_12
Abstrakt:	Protein microarrays are emerging tools which have become very powerful in multiplexed detection technologies. A variety of proteins can be immobilized on a sensor chip allowing for multiplexed diagnostics. Therefore, various types of analyte in a small volume of sample can be detected simultaneously. Protein immobilization is a crucial step for creating a robust and sensitive protein microarray-based detection system. In order to achieve a successful protein immobilization and preserve the activity of the proteins after immobilization, DNA-directed immobilization is a promising technique. Here, we present the design and the use of DNA-directed immobilized (DDI) antibodies in fabrication of robust protein microarrays. We focus on application of protein microarrays for capturing and detecting nanoparticles such as intact viruses. Experimental results on Single-particle interferometric reflectance imaging sensor (SP-IRIS) are used to validate the advantages of the DDI method.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu