Low level laser therapy modulates viability, alkaline phosphatase and matrix metalloproteinase-2 activities of osteoblasts.

Autor: Oliveira FA; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., Matos AA; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., Matsuda SS; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., Buzalaf MA; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., Bagnato VS; Departamento de Física e Ciência dos Materiais, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil., Machado MA; Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., Damante CA; Department of Prosthodontics/Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil., Oliveira RC; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil. Electronic address: rodrigocardoso@usp.br., Peres-Buzalaf C; Universidade do Sagrado Coração, Pró-Reitoria de Pesquisa e Pós-Graduação, Bauru, SP, Brazil.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2017 Apr; Vol. 169, pp. 35-40. Date of Electronic Publication: 2017 Feb 28.
DOI: 10.1016/j.jphotobiol.2017.02.020
Abstrakt: Low level laser therapy (LLLT) has been shown to stimulate bone cell metabolism but their impact on the matrix metalloproteinase (MMP) expression and activity is little explored. This study evaluated the influence of LLLT at two different wavelengths, red and infrared, on MC3T3-E1 preosteoblast viability, alkaline phosphatase (ALP) and MMP-2 and -9 activities. To accomplish this, MC3T3-E1 cells were irradiated with a punctual application of either red (660nm; InGaAIP active medium) or infrared (780nm; GaAlAs active medium) lasers both at a potency of 20mW, energy dose of 0.08 or 0.16J, and energy density of 1.9J/cm 2 or 3.8J/cm 2 , respectively. The control group received no irradiation. Cellular viability, ALP and MMP-2 and -9 activities were assessed by MTT assay, enzymatic activity and zymography, respectively, at 24, 48 and 72h. The treatment of cells with both red and infrared lasers significantly increased the cellular viability compared to the non-irradiated control group at 24 and 48h. The ALP activity was also up modulated in infrared groups at 24 and 72h, depending on the energy densities. In addition, the irradiation with red laser at the energy density of 1.9J/cm 2 promoted an enhancement of MMP-2 activity at 48 and 72h. However, no differences were observed for the MMP-9 activity. In conclusion, when used at these specific parameters, LLL modulates both preosteoblast viability and differentiation highlighted by the increased ALP and MMP-2 activities induced by irradiation.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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