Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis.

Autor: Kontostathi G; Biotechnology Division, Biomedical Research Foundation, Academy of Athens (BRFAA), Athens, Greece; Laboratory of Biology, University of Athens School of Medicine, Athens, Greece., Zoidakis J; Biotechnology Division, Biomedical Research Foundation, Academy of Athens (BRFAA), Athens, Greece., Makridakis M; Biotechnology Division, Biomedical Research Foundation, Academy of Athens (BRFAA), Athens, Greece., Lygirou V; Biotechnology Division, Biomedical Research Foundation, Academy of Athens (BRFAA), Athens, Greece; Laboratory of Biology, University of Athens School of Medicine, Athens, Greece., Mermelekas G; Biotechnology Division, Biomedical Research Foundation, Academy of Athens (BRFAA), Athens, Greece., Papadopoulos T; Institut National de la Santé et de la Recherche Médicale (INSERM), U1048, Institute of Cardiovascular and Metabolic Disease, Toulouse, France; Université Toulouse III Paul-Sabatier, Toulouse, France., Vougas K; Biotechnology Division, Biomedical Research Foundation, Academy of Athens (BRFAA), Athens, Greece., Vlamis-Gardikas A; Department of Chemistry, Division of Organic Chemistry, Biochemistry and Natural Products, University of Patras, 26504 Rion, Greece., Drakakis P; First Department of Obstetrics and Gynecology, University of Athens School of Medicine, Alexandra Hospital, Athens, Greece., Loutradis D; First Department of Obstetrics and Gynecology, University of Athens School of Medicine, Alexandra Hospital, Athens, Greece., Vlahou A; Biotechnology Division, Biomedical Research Foundation, Academy of Athens (BRFAA), Athens, Greece., Anagnou NP; Laboratory of Biology, University of Athens School of Medicine, Athens, Greece; Laboratory of Cell and Gene Therapy, Biomedical Research Foundation, Academy of Athens, Athens, Greece., Pappa KI; First Department of Obstetrics and Gynecology, University of Athens School of Medicine, Alexandra Hospital, Athens, Greece; Laboratory of Cell and Gene Therapy, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2017; Vol. 2017, pp. 4180703. Date of Electronic Publication: 2017 Feb 02.
DOI: 10.1155/2017/4180703
Abstrakt: Cancer cells acquire unique secretome compositions that contribute to tumor development and metastasis. The aim of our study was to elucidate the biological processes involved in cervical cancer, by performing a proteomic analysis of the secretome from the following informative cervical cell lines: SiHa (HPV16+), HeLa (HPV18+), C33A (HPV-), and HCK1T (normal). Proteins were analyzed by 2D gel electrophoresis coupled to MALDI-TOF-MS. Enrichment of secreted proteins with characteristic profiles for each cell line was followed by the identification of differentially expressed proteins. Particularly, transforming growth factor-beta-induced protein ig-h3 (Beta ig-h3) and peroxiredoxin-2 (PRDX2) overexpression in the secretome of cancer cell lines was detected and confirmed by Western blot. Bioinformatics analysis identified the transcription factor NRF2 as a regulator of differentially expressed proteins in the cervical cancer secretome. NRF2 levels were measured by both Western blot and Multiple Reaction Monitoring (MRM) in the total cell extract of the four cell lines. NRF2 was upregulated in SiHa and C33A compared to HCK1T. In conclusion, the secreted proteins identified in cervical cancer cell lines indicate that aberrant NRF2-mediated oxidative stress response (OSR) is a prominent feature of cervical carcinogenesis.
Competing Interests: The authors declare that there is no conflict of interests regarding the publication of this paper.
Databáze: MEDLINE
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