Elevated hematocrit enhances platelet accumulation following vascular injury.

Autor:	Walton BL; Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC., Lehmann M; Department of Chemical and Biological Engineering, Colorado School of Mines, Golden, CO., Skorczewski T; Department of Mathematics and Statistics, Cornell College, Mount Vernon, IA., Holle LA; Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC., Beckman JD; Division of Hematology/Oncology, Department of Medicine., Cribb JA; Department of Physics and Astronomy, and., Mooberry MJ; Division of Hematology/Oncology, Department of Medicine., Wufsus AR; Department of Chemical and Biological Engineering, Colorado School of Mines, Golden, CO., Cooley BC; McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC., Homeister JW; Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC., Pawlinski R; Division of Hematology/Oncology, Department of Medicine.; McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC., Falvo MR; Department of Physics and Astronomy, and., Key NS; Division of Hematology/Oncology, Department of Medicine.; McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC., Fogelson AL; Department of Mathematics and.; Department of Bioengineering, University of Utah, Salt Lake City, UT; and., Neeves KB; Department of Chemical and Biological Engineering, Colorado School of Mines, Golden, CO.; Department of Pediatrics, University of Colorado-Denver, Aurora, CO., Wolberg AS; Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC.; McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC.
Jazyk:	angličtina
Zdroj:	Blood [Blood] 2017 May 04; Vol. 129 (18), pp. 2537-2546. Date of Electronic Publication: 2017 Mar 01.
DOI:	10.1182/blood-2016-10-746479
Abstrakt:	Red blood cells (RBCs) demonstrate procoagulant properties in vitro, and elevated hematocrit is associated with reduced bleeding and increased thrombosis risk in humans. These observations suggest RBCs contribute to thrombus formation. However, effects of RBCs on thrombosis are difficult to assess because humans and mice with elevated hematocrit typically have coexisting pathologies. Using an experimental model of elevated hematocrit in healthy mice, we measured effects of hematocrit in 2 in vivo clot formation models. We also assessed thrombin generation, platelet-thrombus interactions, and platelet accumulation in thrombi ex vivo, in vitro , and in silico. Compared with controls, mice with elevated hematocrit (RBC HIGH ) formed thrombi at a faster rate and had a shortened vessel occlusion time. Thrombi in control and RBC HIGH mice did not differ in size or fibrin content, and there was no difference in levels of circulating thrombin-antithrombin complexes. In vitro, increasing the hematocrit increased thrombin generation in the absence of platelets; however, this effect was reduced in the presence of platelets. In silico, direct numerical simulations of whole blood predicted elevated hematocrit increases the frequency and duration of interactions between platelets and a thrombus. When human whole blood was perfused over collagen at arterial shear rates, elevating the hematocrit increased the rate of platelet deposition and thrombus growth. These data suggest RBCs promote arterial thrombosis by enhancing platelet accumulation at the site of vessel injury. Maintaining a normal hematocrit may reduce arterial thrombosis risk in humans. (© 2017 by The American Society of Hematology.)
Databáze:	MEDLINE
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