Analysis of Soluble Proteins in Natural Cordyceps sinensis from Different Producing Areas by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Two-dimensional Electrophoresis.

Autor:	Li CH; Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China., Zuo HL; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China., Zhang Q; Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China., Wang FQ; Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China., Hu YJ; State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China., Qian ZM; Sunshine lake Pharma Co., Ltd., Guangdong 523850, China., Li WJ; Sunshine lake Pharma Co., Ltd., Guangdong 523850, China., Xia ZN; Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China., Yang FQ; Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China.
Jazyk:	angličtina
Zdroj:	Pharmacognosy research [Pharmacognosy Res] 2017 Jan-Mar; Vol. 9 (1), pp. 34-38.
DOI:	10.4103/0974-8490.199782
Abstrakt:	Background: As one of the bioactive components in Cordyceps sinensis (CS), proteins were rarely used as index components to study the correlation between the protein components and producing areas of natural CS. Objective: Protein components of 26 natural CS samples produced in Qinghai, Tibet, and Sichuan provinces were analyzed and compared to investigate the relationship among 26 different producing areas. Materials and Methods: Proteins from 26 different producing areas were extracted by Tris-HCl buffer with Triton X-100, and separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE). Results: The SDS-PAGE results indicated that the number of protein bands and optical density curves of proteins in 26 CS samples was a bit different. However, the 2-DE results showed that the numbers and abundance of protein spots in protein profiles of 26 samples were obviously different and showed certain association with producing areas. Conclusions: Based on the expression values of matched protein spots, 26 batches of CS samples can be divided into two main categories (Tibet and Qinghai) by hierarchical cluster analysis. Summary: The number of protein bands and optical density curves of proteins in 26 Cordyceps sinensis samples were a bit different on the sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profilesNumbers and abundance of protein spots in protein profiles of 26 samples were obvious different on two-dimensional electrophoresis mapsTwenty-six different producing areas of natural Cordyceps sinensis samples were divided into two main categories (Tibet and Qinghai) by Hierarchical cluster analysis based on the values of matched protein spots. Abbreviations Used : SDS-PAGE: Sodium dodecyl sulfate polyacrylamide gel electrophoresis, 2-DE: Two-dimensional electrophoresis, Cordyceps sinensis : CS, TCMs: Traditional Chinese medicines. Competing Interests: There are no conflicts of interest.
Databáze:	MEDLINE
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