Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers.

Autor: Fuller FD; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Gul S; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Chatterjee R; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Burgie ES; Department of Biology, Washington University in St. Louis, St. Louis, Missouri, USA., Young ID; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Lebrette H; Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden., Srinivas V; Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden., Brewster AS; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Michels-Clark T; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Clinger JA; Department of BioSciences, Rice University, Houston, Texas, USA., Andi B; National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, New York, USA., Ibrahim M; Institut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany., Pastor E; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., de Lichtenberg C; Institutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden., Hussein R; Institut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany., Pollock CJ; Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA., Zhang M; Institut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany., Stan CA; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Kroll T; SSRL, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Fransson T; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Weninger C; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Kubin M; Institute for Methods and Instrumentation on Synchrotron Radiation Research, Helmholtz Zentrum Berlin für Materialien und Energie GmbH, Berlin, Germany., Aller P; Diamond Light Source Limited, Harwell Science and Innovation Campus, Didcot, UK., Lassalle L; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Bräuer P; Diamond Light Source Limited, Harwell Science and Innovation Campus, Didcot, UK.; Department of Biochemistry, University of Oxford, Oxford, UK., Miller MD; Department of BioSciences, Rice University, Houston, Texas, USA., Amin M; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Koroidov S; Institutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden.; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Roessler CG; National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, New York, USA., Allaire M; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Sierra RG; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Docker PT; Diamond Light Source Limited, Harwell Science and Innovation Campus, Didcot, UK., Glownia JM; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Nelson S; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Koglin JE; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Zhu D; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Chollet M; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Song S; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Lemke H; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Liang M; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Sokaras D; SSRL, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Alonso-Mori R; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Zouni A; Institut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany., Messinger J; Institutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden.; Department of Chemistry-Ångström, Molecular Biomimetics, Uppsala University, Uppsala, Sweden., Bergmann U; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Boal AK; Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania, USA., Bollinger JM Jr; Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania, USA., Krebs C; Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania, USA., Högbom M; Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.; Department of Chemistry, Stanford University, Stanford, California, USA., Phillips GN Jr; Department of BioSciences, Rice University, Houston, Texas, USA.; Department of Chemistry, Rice University, Houston, Texas, USA., Vierstra RD; Department of Biology, Washington University in St. Louis, St. Louis, Missouri, USA., Sauter NK; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Orville AM; Diamond Light Source Limited, Harwell Science and Innovation Campus, Didcot, UK., Kern J; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.; LCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA., Yachandra VK; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Yano J; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Jazyk: angličtina
Zdroj: Nature methods [Nat Methods] 2017 Apr; Vol. 14 (4), pp. 443-449. Date of Electronic Publication: 2017 Feb 27.
DOI: 10.1038/nmeth.4195
Abstrakt: X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem II, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.
Databáze: MEDLINE
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