Molecular mechanisms of apoptosis in hepatocellular carcinoma cells induced by ethanol extracts of Solanum lyratum Thumb through the mitochondrial pathway.

Autor:	Mo XQ; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Wei HY; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Huang GR; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Xu LY; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Chen YL; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Qi J; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Xian W; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Qin YC; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Wei LD; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Zhao LJ; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Huang YQ; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Xing W; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Pu HQ; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Wei PY; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Li CG; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China., Liang QC; Xiao-Qiang Mo, Hong-Yu Wei, Gan-Rong Huang, Yu-Li Chen, Wei Xian, Yan-Chun Qin, Lian-Deng Wei, Li-Juan Zhao, Yan-Qiang Huang, Wei Xing, Hong-Qin Pu, Peng-Ya Wei, Chao-Gan Li, Qiu-chun Liang, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China.
Jazyk:	angličtina
Zdroj:	World journal of gastroenterology [World J Gastroenterol] 2017 Feb 14; Vol. 23 (6), pp. 1010-1017.
DOI:	10.3748/wjg.v23.i6.1010
Abstrakt:	Aim: To explore the induction effects and mechanism of Solanum lyratum Thumb (ST) on human hepatocellular carcinoma SMMC-7721 cells through the mitochondrial pathway. Methods: The experiments were conducted on three groups: an experimental group (with ST ethanol extracts' concentration being 2.5, 5 and 10 mg/L), a negative control group (with only nutrient solution, 0 mg/L ST ethanol extracts), and a positive control group (2.5 mg/L DDP). The inhibition rate of cell proliferation was checked by using the methyl thiazolyl tetrazolium method, and cell apoptosis was tested by TUNEL method. Furthermore, RT-PCR was used to examine mRNA expression of Fas, FasL, caspase-8, caspase-3, p53 and Bcl-2 genes. Results: Compared with the negative control group, the inhibition and apoptosis rates of the experimental group with different concentrations of ST extracts on human hepatocellular carcinoma SMMC-7721 cells significantly increased ( P < 0.05). Besides, the mRNA expression of FasL and Bcl-2 significantly decreased ( P < 0.05) while the mRNA expression of Fas, caspase-8, caspase-3 and p53 increased significantly. When compared with the positive control group, the experimental groups with 5 mg/L ST ethanol extracts showed effects similar to the positive control group. Conclusion: ST ethanol extracts induced the apoptosis of hepatocellular carcinoma SMMC-7721 cells through up-regulated Fas, caspase-8, caspse-3 and p53, and down-regulated FasL and Bcl-2 in the mitochondrial pathway. Competing Interests: Conflict-of-interest statement: We declare that there are no conflicts of interest to disclose.
Databáze:	MEDLINE
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