| Autor: |
Nizyaeva NV; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia., Nagovitsyna MN; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia., Kulikova GV; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia., Tumanova UN; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia., Poltavtseva RA; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia., Fedorova IA; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia., Yushina MN; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia., Pavlovich SV; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia., Shchyogolev AI; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health of the Russian Federation, Moscow, Russia. ashegolev@oparina4.ru. |
| Abstrakt: |
Placentas from women aged 25-32 years with normal course of gestation were studied. It is essential to stick to certain methodological approaches for preparing viable multipotent mesenchymal stromal cell culture and to carry out morphological (macro and micro) evaluation of the chorionic villi, umbilical cords, and placentas. At stage I of the study, patients' histories, labor course, and examinations of the newborns should be analyzed to exclude women with genital and extragenital diseases. At stage II, it is essential to stick to special regulations and methods for collection of specimens of the cord, amnion, and placental tissue proper. Histological control of the placental structures collected for multipotent mesenchymal stromal cell culturing is obligatory. |
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