Rapid Molecular Detection of Zika Virus in Acute-Phase Urine Samples Using the Recombinase Polymerase Amplification Assay.

Autor:	Abd El Wahed A; Division of Microbiology and Animal Hygiene, Institute of Veterinary Medicine, Department of Animal Sciences, Georg-August-University, Goettingen, Lower Saxony, Germany., Sanabani SS; Hospital das Clínicas, School of Medicine, University of São Paulo, São Paulo, Brazil., Faye O; Arbovirus and viral hemorragic fever unit, Institut Pasteur de Dakar, Dakar, Senegal., Pessôa R; Hospital das Clínicas, School of Medicine, University of São Paulo, São Paulo, Brazil., Patriota JV; Municipal Hospital of Tuparetama, Tuparetama, Pernambuco, Brazil., Giorgi RR; University of Santo Amaro, São Paulo, São Paulo, Brazil., Patel P; TIB MOLBIOL Syntheselabor GmbH, Berlin, Germany., Böhlken-Fascher S; Georg-August-University Goettingen., Landt O; TIB MOLBIOL Syntheselabor GmbH, Berlin, Germany., Niedrig M; Robert Koch Institut Robert Koch Institut., Zanotto PM; Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Biomedical Sciences Institute, University of São Paulo, São Paulo, Brazil., Czerny CP; Division of Microbiology and Animal Hygiene, Institute of Veterinary Medicine, Department of Animal Sciences, Georg-August-University, Goettingen, Lower Saxony, Germany., Sall AA; Arbovirus and viral hemorragic fever unit, Institut Pasteur de Dakar, Dakar, Senegal., Weidmann M
Jazyk:	angličtina
Zdroj:	PLoS currents [PLoS Curr] 2017 Jan 25; Vol. 9. Date of Electronic Publication: 2017 Jan 25.
DOI:	10.1371/currents.outbreaks.a7f1db2c7d66c3fc0ea0a774305d319e
Abstrakt:	Background: Currently the detection of Zika virus (ZIKV) in patient samples is done by real-time RT-PCR. Samples collected from rural area are sent to highly equipped laboratories for screening. A rapid point-of-care test is needed to detect the virus, especially at low resource settings. Methodology/principal Findings: In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV. RT-RPA assay was portable, sensitive (21 RNA molecules), and rapid (3-15 minutes). No cross-reactivity was detected to other flaviviruses, alphaviruses and arboviruses. Compared to real-time RT-PCR, the diagnostic sensitivity was 92%, while the specificity was 100%. Conclusions/significance: The developed assay is a promising platform for rapid point of need detection of ZIKV in low resource settings and elsewhere (e.g. during mass gathering).
Databáze:	MEDLINE
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