| Autor: |
Rauwolf M; Atominstitut TU Wien Vienna Austria., Pemmer B; Atominstitut TU Wien Vienna Austria., Roschger A; 1st Med. Department Hanusch Hospital Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling Vienna Austria., Turyanskaya A; Atominstitut TU Wien Vienna Austria., Smolek S; Atominstitut TU Wien Vienna Austria., Maderitsch A; Atominstitut TU Wien Vienna Austria., Hischenhuber P; Atominstitut TU Wien Vienna Austria., Foelser M; Atominstitut TU Wien Vienna Austria., Simon R; ANKA synchrotron radiation source Karlsruhe Institute of Technology (KIT) Eggenstein-Leopoldshafen Germany., Lang S; Department of Pathology Vienna General Hospital, Medical University of Vienna Vienna Austria., Puchner SE; Department of Orthopaedic Surgery Vienna General Hospital, Medical University of Vienna Vienna Austria., Windhager R; Department of Orthopaedic Surgery Vienna General Hospital, Medical University of Vienna Vienna Austria., Klaushofer K; 1st Med. Department Hanusch Hospital Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling Vienna Austria., Wobrauschek P; Atominstitut TU Wien Vienna Austria., Hofstaetter JG; Orthopaedic Hospital Vienna-Speising Vienna Austria; 1st Med. Department Hanusch Hospital Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling Vienna Austria., Roschger P; 1st Med. Department Hanusch Hospital Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling Vienna Austria., Streli C; Atominstitut TU Wien Vienna Austria. |
| Abstrakt: |
Abnormal tissue levels of certain trace elements such as zinc (Zn) were reported in various types of cancer. Little is known about the role of Zn in osteosarcoma. Using confocal synchrotron radiation micro X-ray fluorescence analysis, we characterized the spatial distribution of Zn in high-grade sclerosing osteosarcoma of nine patients (four women/five men; seven knee/one humerus/one femur) following chemotherapy and wide surgical resection. Levels were compared with adjacent normal tissue. Quantitative backscattered electron imaging as well as histological examinations was also performed. On average, the ratio of medians of Zn count rates (normalized to calcium) in mineralized tumor tissue was about six times higher than in normal tissue. There was no difference in Zn levels between tumor fraction areas with a low fraction and a high fraction of mineralized tissue, which were clearly depicted using quantitative backscattered electron imaging. Moreover, we found no correlation between the Zn values and the type of tumor regression according to the Salzer-Kuntschik grading. The underlying mechanism of Zn accumulation remains unclear. Given the emerging data on the role of trace elements in other types of cancer, our novel results warrant further studies on the role of trace elements in bone cancer. Copyright © 2016 The Authors. X-Ray Spectrometry published by John Wiley & Sons Ltd. |
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