| Autor: |
Abdelatty AM; Animal and Avian Sciences Department, University of Maryland, College Park 20742; Nutrition and Clinical Nutrition Department, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt 12211., Iwaniuk ME; Animal and Avian Sciences Department, University of Maryland, College Park 20742., Garcia M; Animal and Avian Sciences Department, University of Maryland, College Park 20742., Moyes KM; Animal and Avian Sciences Department, University of Maryland, College Park 20742., Teter BB; Animal and Avian Sciences Department, University of Maryland, College Park 20742., Delmonte P; Food and Drug Administration, College Park, MD 20740., Kadegowda AKG; Animal and Avian Sciences Department, University of Maryland, College Park 20742., Tony MA; Nutrition and Clinical Nutrition Department, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt 12211., Mohamad FF; Nutrition and Clinical Nutrition Department, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt 12211., Erdman RA; Animal and Avian Sciences Department, University of Maryland, College Park 20742. Electronic address: erdman@umd.edu. |
| Abstrakt: |
Investigations of the temporal changes in mammary gene expression that occur during sudden diet change have been limited by the use of mammary tissue as the source of RNA because of the invasive nature of mammary biopsy procedures. However, the cytosolic crescent, present in 1% of the largest milk fat globules, contains mammary epithelial cell RNA that has become trapped between the inner and outer milk fat globule membranes during final formation and secretion of milk fat into the lumen of the mammary alveoli. We hypothesized that cytosolic crescent RNA extracted from milk fat could be used as an alternative source of mammary epithelial cell RNA to measure the immediate temporal changes in gene expression as a result of changes in diet. In this experiment, feed restriction was used to mimic the state of negative energy balance observed in early lactation and induce a rapid change in milk fat yield and lipogenic gene expression. Ten multiparous Holstein dairy were fed a basal diet ad libitum during a 14-d preliminary period followed by a 4-d experimental period where 5 cows remained on ad libitum feeding and 5 cows were fed at 60% of their d 8-14 intakes (restricted) on d 15 to 18 and then returned to ad libitum feeding on d 19 to 21. Milk samples were collected from each milking on d 13 to 20 and the milk fat was immediately isolated, mixed with Trizol LS, and stored at -80°C for subsequent extraction of RNA that was used for measurement of gene expression. Feed restriction tended to increase milk fat percentage. However, total milk and milk fat production were reduced by 21 and 18%, respectively. Consistent with increased use of body fat for milk synthesis, serum nonesterified fatty acids increased 6-fold (0.78 mEq/L in the feed restriction vs. 0.13 mEq/L ad libitum group), whereas the milk fatty acids (Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.) |
