Genomic array as compared to karyotyping in myelodysplastic syndromes in a prospective clinical trial.

Autor: Stevens-Kroef MJ; Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands., Olde Weghuis D; Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands., ElIdrissi-Zaynoun N; Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands., van der Reijden B; Department of Laboratory Medicine, Radboud University Medical Center, Nijmegen, The Netherlands., Cremers EMP; Department of Hematology, VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands., Alhan C; Department of Hematology, VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands., Westers TM; Department of Hematology, VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands., Visser-Wisselaar HA; Department of Hematology, Erasmus MC Cancer Institute, HOVON Data Center, Erasmus University Medical Center-Daniel den Hoed, Rotterdam, The Netherlands., Chitu DA; Department of Hematology, Erasmus MC Cancer Institute, HOVON Data Center, Erasmus University Medical Center-Daniel den Hoed, Rotterdam, The Netherlands., Cunha SM; Department of Hematology, Erasmus MC Cancer Institute, HOVON Data Center, Erasmus University Medical Center-Daniel den Hoed, Rotterdam, The Netherlands., Vellenga E; Department of Experimental Hematology, University Medical Center Groningen, Groningen, The Netherlands., Klein SK; Department of Internal Medicine, Meander Medisch Centrum, Amersfoort, The Netherlands., Wijermans P; Department of Internal Medicine, Haga Ziekenhuis, The Hague, The Netherlands., de Greef GE; Department of Hematology, Erasmus University Medical Center-Daniel den Hoed, Rotterdam, The Netherlands., Schaafsma MR; Department of Internal Medicine, Medisch Centrum Twente, Enschede, The Netherlands., Muus P; Department of Hematology, Radboud University Medical Center, Nijmegen, The Netherlands., Ossenkoppele GJ; Department of Hematology, VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands., van de Loosdrecht AA; Department of Hematology, VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands., Jansen JH; Department of Laboratory Medicine, Radboud University Medical Center, Nijmegen, The Netherlands.
Jazyk: angličtina
Zdroj: Genes, chromosomes & cancer [Genes Chromosomes Cancer] 2017 Jul; Vol. 56 (7), pp. 524-534. Date of Electronic Publication: 2017 Mar 31.
DOI: 10.1002/gcc.22455
Abstrakt: Karyotyping is considered as the gold standard in the genetic subclassification of myelodysplastic syndrome (MDS). Oligo/SNP-based genomic array profiling is a high-resolution tool that also enables genome wide analysis. We compared karyotyping with oligo/SNP-based array profiling in 104 MDS patients from the HOVON-89 study. Oligo/SNP-array identified all cytogenetically defined genomic lesions, except for subclones in two cases and balanced translocations in three cases. Conversely, oligo/SNP-based genomic array profiling had a higher success rate, showing 55 abnormal cases, while an abnormal karyotype was found in only 35 patients. In nine patients whose karyotyping was unsuccessful because of insufficient metaphases or failure, oligo/SNP-based array analysis was successful. Based on cytogenetic visible abnormalities as identified by oligo/SNP-based genomic array prognostic scores based on IPSS/-R were assigned. These prognostic scores were identical to the IPSS/-R scores as obtained with karyotyping in 95%-96% of the patients. In addition to the detection of cytogenetically defined lesions, oligo/SNP-based genomic profiling identified focal copy number abnormalities or regions of copy neutral loss of heterozygosity that were out of the scope of karyotyping and fluorescence in situ hybridization. Of interest, in 26 patients we demonstrated such cytogenetic invisible abnormalities. These abnormalities often involved regions that are recurrently affected in hematological malignancies, and may therefore be of clinical relevance. Our findings indicate that oligo/SNP-based genomic array can be used to identify the vast majority of recurrent cytogenetic abnormalities in MDS. Furthermore, oligo/SNP-based array profiling yields additional genetic abnormalities that may be of clinical importance.
(© 2017 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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