Single-molecule FRET unveils induced-fit mechanism for substrate selectivity in flap endonuclease 1.

Autor:	Rashid F; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Harris PD; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Zaher MS; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Sobhy MA; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Joudeh LI; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Yan C; Department of Chemistry, Georgia State University, Atlanta, United States.; Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, United States., Piwonski H; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Tsutakawa SE; Lawrence Berkeley National Laboratory, Berkeley, United States., Ivanov I; Department of Chemistry, Georgia State University, Atlanta, United States.; Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, United States., Tainer JA; Lawrence Berkeley National Laboratory, Berkeley, United States.; Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, United States., Habuchi S; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Hamdan SM; Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2017 Feb 23; Vol. 6. Date of Electronic Publication: 2017 Feb 23.
DOI:	10.7554/eLife.21884
Abstrakt:	Human flap endonuclease 1 (FEN1) and related structure-specific 5'nucleases precisely identify and incise aberrant DNA structures during replication, repair and recombination to avoid genomic instability. Yet, it is unclear how the 5'nuclease mechanisms of DNA distortion and protein ordering robustly mediate efficient and accurate substrate recognition and catalytic selectivity. Here, single-molecule sub-millisecond and millisecond analyses of FEN1 reveal a protein-DNA induced-fit mechanism that efficiently verifies substrate and suppresses off-target cleavage. FEN1 sculpts DNA with diffusion-limited kinetics to test DNA substrate. This DNA distortion mutually 'locks' protein and DNA conformation and enables substrate verification with extreme precision. Strikingly, FEN1 never misses cleavage of its cognate substrate while blocking probable formation of catalytically competent interactions with noncognate substrates and fostering their pre-incision dissociation. These findings establish FEN1 has practically perfect precision and that separate control of induced-fit substrate recognition sets up the catalytic selectivity of the nuclease active site for genome stability.
Databáze:	MEDLINE
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