Quercetin exerts preventive, ameliorative and prophylactic effects on cadmium chloride - induced oxidative stress in the uterus and ovaries of female Wistar rats.

Autor: Nna VU; Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria; Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia. Electronic address: victor2nna@gmail.com., Usman UZ; Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, Kelantan, Malaysia; Usmanu Danfodiyo University, Sokoto State, Nigeria., Ofutet EO; Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria., Owu DU; Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Cross River State, Nigeria.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2017 Apr; Vol. 102, pp. 143-155. Date of Electronic Publication: 2017 Feb 14.
DOI: 10.1016/j.fct.2017.02.010
Abstrakt: This study examined the possible protective effect of quercetin(QE) on cadmium chloride (CdCl 2 ) - induced reproductive toxicity in female rats. Cadmium (Cd) accumulated in the uterus and ovaries of rats, decreased antioxidants [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione (GSH)], and raised the concentrations of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) in the uterus and ovaries of rats. Serum concentrations of estradiol, progesterone, follicle stimulating hormone and luteinizing hormone decreased significantly after CdCl 2 administration. Caspase-3 activity significantly increased in the ovaries, with an increase in Bax and a decrease in Bcl-2 protein expressions after CdCl 2 treatment. Histopathology of the ovaries revealed significant decrease in follicle number, while the uterus showed cyst-like endometrial glands. All three models of QE treatment [pre-treatment (QE + CdCl 2 ), post-treatment (CdCl 2 +QE), simultaneous treatment (CdCl 2 /QE)] decreased Cd accumulation, MDA, H 2 O 2 , and increased SOD, CAT and GPx activities in the uterus and ovaries, decreased apoptosis of follicular cells, and increased serum reproductive hormones. However, the QE pre-treated model offered better protection against CdCl 2 relative to the other two models. These results suggest that, QE exerts multi-mechanistic protective effects against cadmium toxicity attributable to its antioxidant and anti-apoptotic actions.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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