Effects of duodenal-jejunal bypass on structure of diaphragm in western diet obese rats.

Autor:	Ulsenheimer BH; Fellow Master degree, Postgraduate Program in Biosciences and Health, Universidade Estadual do Oeste do Paraná (UNIOESTE), Cascavel-PR, Brazil. Scholarship research from CAPES and Araucaria Foundation. Acquisition and interpretation of data, statistical analysis., Confortim HD; Fellow Master degree, Postgraduate Program in Biosciences and Health, UNIOESTE, Cascavel-PR, Brazil. Technical procedures., Jeronimo LC; Fellow Master degree, Postgraduate Program in Biosciences and Health, UNIOESTE, Cascavel-PR, Brazil. Technical procedures., Centenaro LA; PhD, Assistant Professor, Center of Medical and Pharmaceutical Sciences, UNIOESTE, Cascavel-PR, Brazil. Conception, design, intellectual and scientific content of the study; manuscript writing., Guimarães AT; PhD, Assistant Professor, Center for Biological Sciences and Health, UNIOESTE, Cascavel-PR, Brazil. Statistical analysis., Bonfleur ML; PhD, Assistant Professor, Human Physiology Division, Center for Biological Sciences and Health, UNIOESTE, Cascavel-PR, Brazil. Conception, intellectual and scientific content of the study; manuscript writing., Balbo SL; PhD, Assistant Professor, Human Physiology Division, Center for Biological Sciences and Health, UNIOESTE, Cascavel-PR Brazil. Intellectual and scientific content of the study., Matheus SM; PhD, Assistant Professor, Department of Anatomy, Institute of Biosciences, Universidade Estadual Paulista (UNESP), Botucatu-SP, Brazil. Transmission electron microscope analysis., Torrejais MM; PhD, Assistant Professor, Center of Medical and Pharmaceutical Sciences, UNIOESTE, Cascavel-PR, Brazil. Conception, design, intellectual and scientific content of the study; technical procedures.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2017 Jan; Vol. 32 (1), pp. 1-13.
DOI:	10.1590/s0102-865020170101
Abstrakt:	Purpose:: To evaluate the effects of duodenal-jejunal bypass (DJB) on the diaphragm muscle of obese rats fed on a western diet (WD) . Methods:: Eighteen male Wistar rats were fed a standard rodent chow diet (CTL group) or WD ad libitum. After 10 weeks, WD rats were submitted to sham (WD SHAM) or duodenal-jejunal bypass (WD DJB). The structure, ultrastructure, collagen content and the morphometry of the neuromuscular junctions (NMJs) were analyzed two months after surgery. Results:: WD SHAM rats displayed an increase in body weight, the Lee index and retroperitoneal and peri-epididymal fat pads compared to the CTL group. DJB did not alter these parameters. The muscle fiber structure and NMJs were similar in the WD SHAM and CTL groups. However, the WD SHAM group showed alterations in the fiber ultrastructure, such as loosely arranged myofibrils and Z line disorganization. In addition, WD SHAM animals presented a considerable amount of lipid droplets and a reduction in the percentage of collagen compared to the CTL group. DJB did not affect the structure or ultrastructure of the muscle fibers or the NMJs in the diaphragm of the WD DJB animals. Conclusion:: Duodenal-jejunal bypass did not improve the alterations observed in the diaphragm of western diet obese-rats.
Databáze:	MEDLINE
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