| Autor: |
Betancur LA; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera, Edificio de Química of 427, Bogotá, Colombia.; Universidad de Caldas. Departamento de Química. Edificio Orlando Sierra, Bloque B, Sede Palogrande Calle. Manizales, Caldas, Colombia., Naranjo-Gaybor SJ; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera, Edificio de Química of 427, Bogotá, Colombia.; Universidad de las Fuerzas Armadas, ESPE Carrera de Ingeniería Agropecuaria IASA II Av. General Rumiñahui s/n, Sangolquí- Ecuador., Vinchira-Villarraga DM; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera, Edificio de Química of 427, Bogotá, Colombia., Moreno-Sarmiento NC; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera, Edificio de Química of 427, Bogotá, Colombia., Maldonado LA; Universidad Autónoma Metropolitana Rectoría-Secretaría General, Prolongación Canal de Miramontes, Col. Ex-hacienda San Juan de Dios, Tlalpan, México DF., Suarez-Moreno ZR; Investigación y Desarrollo, Empresa Colombiana de Productos Veterinarios VECOL S.A., Bogotá D.C., Acosta-González A; Universidad de la Sabana, Facultad de Ingeniería, Autopista Norte, Chía, Cundinamarca, Colombia., Padilla-Gonzalez GF; Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Av. do de Sao Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Av. do Café, Ribeirão Preto-SP, Brazil., Puyana M; Departamento de Ciencias Biológicas y Ambientales, Programa de Biología Marina, Universidad Jorge Tadeo Lozano, Carrera, Modulo, Oficina, Bogotá, Colombia., Castellanos L; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera, Edificio de Química of 427, Bogotá, Colombia., Ramos FA; Universidad Nacional de Colombia, Sede Bogotá, Departamento de Química, Carrera, Edificio de Química of 427, Bogotá, Colombia. |
| Abstrakt: |
Marine bacteria are considered as promising sources for the discovery of novel biologically active compounds. In this study, samples of sediment, invertebrate and algae were collected from the Providencia and Santa Catalina coral reef (Colombian Caribbean Sea) with the aim of isolating Actinobateria-like strain able to produce antimicrobial and quorum quenching compounds against pathogens. Several approaches were used to select actinobacterial isolates, obtaining 203 strains from all samples. According to their 16S rRNA gene sequencing, a total of 24 strains was classified within Actinobacteria represented by three genera: Streptomyces, Micromonospora, and Gordonia. In order to assess their metabolic profiles, the actinobacterial strains were grown in liquid cultures, and LC-MS-based analyses from ethyl acetate fractions were performed. Based on taxonomical classification, screening information of activity against phytopathogenic strains and quorum quenching activity, as well as metabolic profiling, six out of the 24 isolates were selected for follow-up with chemical isolation and structure identification analyses of putative metabolites involved in antimicrobial activities. |
