Column switching UHPLC-MS/MS with restricted access material for the determination of CNS drugs in plasma samples.
| Autor: | Acquaro VR Junior; Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., Domingues DS; Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., Costa Queiroz ME; Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Bioanalysis [Bioanalysis] 2017 Mar; Vol. 9 (6), pp. 555-568. Date of Electronic Publication: 2017 Feb 22. |
| DOI: | 10.4155/bio-2016-0301 |
| Abstrakt: | Background: Polypharmacy is a common practice in schizophrenia. Consequently, therapeutic drug monitoring is usually adopted to maintain the concentrations of the drugs in the plasma within a targeted therapeutic range, to maximize therapeutic efficiency and to diminish adverse side effects. Methodology: This study reports on a column switching UHPLC-MS/MS method to determine psychotropic drugs in plasma samples simultaneously. Results: The method was linear from 0.025 to 1.25 ng ml -1 with R 2 above 0.9950 and the lack of fit test (p > 0.05). The precision values presented coefficients of variation lower than 12%, and the relative standard error of the accuracy were lower than 14%. Conclusion: The column switching UHPLC-MS/MS method developed herein successfully determined drugs in schizophrenic patients' plasma samples for therapeutic drug monitoring. |
| Databáze: | MEDLINE |
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