Genomic variability of Helicobacter pylori isolates of gastric regions from two Colombian populations.

Autor:	Matta AJ; Andrés Jenuer Matta, Javier Andrés Bustamante, Luis Eduardo Bravo, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, Cali 760043, Colombia., Pazos AJ; Andrés Jenuer Matta, Javier Andrés Bustamante, Luis Eduardo Bravo, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, Cali 760043, Colombia., Bustamante-Rengifo JA; Andrés Jenuer Matta, Javier Andrés Bustamante, Luis Eduardo Bravo, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, Cali 760043, Colombia., Bravo LE; Andrés Jenuer Matta, Javier Andrés Bustamante, Luis Eduardo Bravo, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, Cali 760043, Colombia.
Jazyk:	angličtina
Zdroj:	World journal of gastroenterology [World J Gastroenterol] 2017 Feb 07; Vol. 23 (5), pp. 800-809.
DOI:	10.3748/wjg.v23.i5.800
Abstrakt:	Aim: To compare the genomic variability and the multiple colonization of Helicobacter pylori ( H. pylori ) in patients with chronic gastritis from two Colombian populations with contrast in the risk of developing gastric cancer (GC): Túquerres-Nariño (High risk) and Tumaco-Nariño (Low risk). Methods: Four hundred and nine patients from both genders with dyspeptic symptoms were studied. Seventy-two patients were included in whom H. pylori was isolated from three anatomic regions of the gastric mucosa, (31/206) of the high risk population of GC (Túquerres) and (41/203) of the low risk population of GC (Tumaco). The isolates were genotyped by PCR-RAPD. Genetic diversity between the isolates was evaluated by conglomerates analysis and multiple correspondence analyses. Results: The proportion of virulent genotypes of H. pylori was 99% in Túquerres and 94% in Tumaco. The coefficient of similarity of Nei-Li showed greater genetic diversity among isolates of Túquerres (0.13) than those of Tumaco (0.07). After adjusting by age, gender and type of gastritis, the multiple colonization was 1.7 times more frequent in Túquerres than in Tumaco ( P = 0.05). Conclusion: In Túquerres, high risk of GC there was a greater probability of multiple colonization by H. pylori . From the analysis of the results of the PCR-RAPD, it was found higher genetic variability in the isolates of H. pylori in the population of high risk for the development of GC. Competing Interests: Conflict-of-interest statement: The authors declare that there is no conflict of interest related to this study.
Databáze:	MEDLINE
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