Antimicrobial resistance and genomic rep-PCR fingerprints of Pseudomonas aeruginosa strains from animals on the background of the global population structure.

Autor: Serrano I; CIISA/Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal., Oliveira M; CIISA/Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal. moliveira@fmv.ulisboa.pt., Santos JP; CIISA/Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal., Bilocq F; Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels, Belgium., Leitão A; CIISA/Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal., Tavares L; CIISA/Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal., Pirnay JP; Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels, Belgium., De Vos D; Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels, Belgium.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2017 Feb 21; Vol. 13 (1), pp. 58. Date of Electronic Publication: 2017 Feb 21.
DOI: 10.1186/s12917-017-0977-8
Abstrakt: Background: Pseudomonas aeruginosa is an important human opportunistic pathogen responsible for fatal nosocomial infections worldwide, and has emerged as a relevant animal pathogen. Treatment options are dramatically decreasing, due to antimicrobial resistance and the microorganism's large versatile genome. Antimicrobial resistance profiles, serotype frequency and genomic profile of unrelated P. aeruginosa isolates of veterinary origin (n = 73), including domesticated, farm, zoo and wild animals mainly from Portugal were studied. The genomic profile, determined by DiversiLab system (Rep-PCR-based technique), was compared with the P. aeruginosa global population structure to evaluate their relatedness.
Results: Around 40% of the isolates expressed serotypes O6 (20.5%) and O1 (17.8%). A total of 46.6% of isolates was susceptible to all antimicrobials tested. Isolates obtained from most animals were non-multidrug resistant (86.3%), whereas 11% were multidrug resistant, MDR (non-susceptible to at least one agent in ≥ three antimicrobial categories), and 2.7% extensively drug resistant, XDR (non-susceptible to at least one agent in all but two or fewer antimicrobial categories). Resistance percentages were as follows: amikacin (0.0%), aztreonam (41.1%), cefepime (9.6%), ceftazidime (2.7%), ciprofloxacin (15.1%), colistin (0.0%), gentamicin (12.3%), imipenem (1.4%), meropenem (1.4%), piperacillin + tazobactam (12.3%), ticarcillin (16.4%), ticarcillin + clavulanic acid (17.8%), and tobramycin (1.4%). Animal isolates form a population with a non-clonal epidemic structure indistinguishable from the global P. aeruginosa population structure, where no specific 'animal clonal lineage' was detected.
Conclusions: Serotypes O6 and O1 were the most frequent. Serotype frequency and antimicrobial resistance patterns found in P. aeruginosa from animals were as expected for this species. This study confirms earlier results that P. aeruginosa has a non-clonal population structure, and shows that P. aeruginosa population from animals is homogeneously scattered and indistinguishable from the global population structure.
Databáze: MEDLINE
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