| Autor: |
Mulumba-Mfumu LK; Central Veterinary Laboratory, Avenue Wangata, P.O. Box 8842, Kinshasa I, Democratic Republic of Congo. labovetkin@yahoo.fr.; Research Unit of Epidemiology and Risk Analysis Applied to Veterinary (UREAR-Ulg), Fundamental and Applied Research for Animals & Health, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium. labovetkin@yahoo.fr., Achenbach JE; Animal Production and Health Laboratory, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, Austria. achenbach@battelle.org., Mauldin MR; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. MMauldin@cdc.gov.; Oak Ridge Institute for Science and Education (ORISE) CDC Fellowship Program, Oak Ridge, TN 37830, USA. MMauldin@cdc.gov., Dixon LK; Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 ONF, UK. linda.dixon@pirbright.ac.uk., Tshilenge CG; Central Veterinary Laboratory, Avenue Wangata, P.O. Box 8842, Kinshasa I, Democratic Republic of Congo. george.tshilenge@sacids.org., Thiry E; Research Unit of Epidemiology and Risk Analysis Applied to Veterinary (UREAR-Ulg), Fundamental and Applied Research for Animals & Health, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium. etienne.thiry@ulg.ac.be., Moreno N; Centro de Investigaciόn en Sanidad Animal (CISA), INIA, Valdeolmos, 28130 Madrid, Spain. moreno.noelia@inia.es., Blanco E; Centro de Investigaciόn en Sanidad Animal (CISA), INIA, Valdeolmos, 28130 Madrid, Spain. blanco@inia.es., Saegerman C; Research Unit of Epidemiology and Risk Analysis Applied to Veterinary (UREAR-Ulg), Fundamental and Applied Research for Animals & Health, Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium. claude.saegerman@ulg.ac.be., Lamien CE; Animal Production and Health Laboratory, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, Austria. c.lamien@iaea.org., Diallo A; Animal Production and Health Laboratory, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, A-1400 Vienna, Austria. adama.diallo@cirad.fr. |
| Abstrakt: |
African swine fever (ASF) is a devastating disease of domestic pigs. It is a socioeconomically important disease, initially described from Kenya, but subsequently reported in most Sub-Saharan countries. ASF spread to Europe, South America and the Caribbean through multiple introductions which were initially eradicated-except for Sardinia-followed by re‑introduction into Europe in 2007. In this study of ASF within the Democratic Republic of the Congo, 62 domestic pig samples, collected between 2005-2012, were examined for viral DNA and sequencing at multiple loci: C-terminus of the B646L gene (p72 protein), central hypervariable region (CVR) of the B602L gene, and the E183L gene (p54 protein). Phylogenetic analyses identified three circulating genotypes: I (64.5% of samples), IX (32.3%), and XIV (3.2%). This is the first evidence of genotypes IX and XIV within this country. Examination of the CVR revealed high levels of intra-genotypic variation, with 19 identified variants. |
