99m Tc-Tetrofosmin Uptake Correlates with the Sensitivity of Glioblastoma Cell Lines to Temozolomide.

Autor: Alexiou GA; Neurosurgical Institute, University of Ioannina, Ioannina, Greece., Xourgia X; Department of Nuclear Medicine, University Hospital of Ioannina, Ioannina, Greece., Gerogianni P; Neurosurgical Institute, University of Ioannina, Ioannina, Greece., Vartholomatos E; Neurosurgical Institute, University of Ioannina, Ioannina, Greece., Kalef-Ezra JA; Department of Medical Physics, University of Ioannina, Ioannina, Greece., Fotopoulos AD; Department of Nuclear Medicine, University Hospital of Ioannina, Ioannina, Greece., Kyritsis AP; Neurosurgical Institute, University of Ioannina, Ioannina, Greece.
Jazyk: angličtina
Zdroj: World journal of nuclear medicine [World J Nucl Med] 2017 Jan-Mar; Vol. 16 (1), pp. 45-50.
DOI: 10.4103/1450-1147.181155
Abstrakt: 99m Tc-tetrofosmin ( 99m Tc-TF) is a single-photon emission computed tomography tracer that has been used for brain tumor imaging. The aim of the study was to assess if 99m Tc-TF uptake by glioblastoma cells correlates with their response to temozolomide (TMZ). We investigated the correlation of TMZ antitumor effect with the 99m Tc-TF uptake in two glioblastoma cell lines. The U251MG cell line is sensitive to TMZ, whereas T98G is resistant. Viability and proliferation of the cells were examined by trypan blue exclusion assay and xCELLigence system. Cell cycle was analyzed with flow cytometry. The radioactivity in the cellular lysate was measured with a gamma scintillation counter. TMZ induced G 2 /M cell cycle arrest in U251MG cells, whereas there was no effect on cell cycle in T98G cells. Lower 99m Tc-TF uptake was observed in U251MG cells that were exposed to TMZ compared to control ( P = 0.0159). No significant difference in respect to 99m Tc-TF uptake was found in T98G cells when exposed to TMZ compared to control ( P = 0.8). With 99m Tc-TF, it was possible to distinguish between TMZ-sensitive and resistant glioblastoma cells within 6 h of treatment initiation. Thus, 99m Tc-TF uptake may consist a novel approach to assess an early response of glioblastoma to chemotherapy and deserves further investigation.
Competing Interests: There are no conflicts of interest.
Databáze: MEDLINE
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