A pilot study of cell-mediated gene therapy for spinal cord injury in mini pigs.

Autor: Islamov RR; Kazan State Medical University, Kazan, Russia., Sokolov ME; Kazan State Medical University, Kazan, Russia; Kazan Federal University, Kazan, Russia., Bashirov FV; Kazan State Medical University, Kazan, Russia., Fadeev FO; Kazan State Medical University, Kazan, Russia; Kazan Federal University, Kazan, Russia., Shmarov MM; Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia., Naroditskiy BS; Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia., Povysheva TV; Kazan State Medical University, Kazan, Russia., Shaymardanova GF; Kazan State Medical University, Kazan, Russia., Yakupov RA; Kazan State Medical Academy, Kazan, Russia., Chelyshev YA; Kazan State Medical University, Kazan, Russia; Kazan Federal University, Kazan, Russia., Lavrov IA; Kazan Federal University, Kazan, Russia; Departments of Neurologic Surgery and Biomedical Engineering, Mayo Clinic, Rochester, MN, United States. Electronic address: lavrov.igor@mayo.edu.
Jazyk: angličtina
Zdroj: Neuroscience letters [Neurosci Lett] 2017 Mar 22; Vol. 644, pp. 67-75. Date of Electronic Publication: 2017 Feb 14.
DOI: 10.1016/j.neulet.2017.02.034
Abstrakt: Currently, in clinical practice there is no efficient way to overcome the sequences of neurodegeneration after spinal cord traumatic injury. Using a new experimental model of spinal cord contusion injury on miniature pigs, we proposed to deliver therapeutic genes encoding vascular endothelial growth factor (VEGF), glial cell line-derived neurotrophic factor (GDNF) and neural cell adhesion molecule (NCAM) to the damaged area, using umbilical cord blood mononuclear cells (UCBC). In this study, genetically engineered UCBC (2×10 6 cells in 200 ml of saline) were injected intrathecally to mini-pigs 10days after SCI. Control and experimental mini pigs were observed for 60days after surgery. Histological, electrophysiological, and clinical evaluation demonstrated significant improvement in animal treated with genetically engineered UCBCs. Difference in recovery of the somatosensory evoked potentials and in histological findings in control and treated animals support the positive effect of the gene-cell constriction for recovery after spinal cord injury. Results of this study suggest that transplantation of UCBCs simultaneously transduced with three recombinant adenoviruses Ad5-VEGF, Ad5-GDNF and Ad5-NCAM represent a novel potentially successful approach for treatment of spinal cord injury.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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