| Autor: |
Wlodarski M; Biological and Soft Systems, Cavendish Laboratory, University of Cambridge, United Kingdom., Raciti B, Kotar J, Cosentino Lagomarsino M, Fraser GM, Cicuta P |
| Jazyk: |
angličtina |
| Zdroj: |
Physical biology [Phys Biol] 2017 Feb 16; Vol. 14 (1), pp. 015005. Date of Electronic Publication: 2017 Feb 16. |
| DOI: |
10.1088/1478-3975/aa5b71 |
| Abstrakt: |
While the action of many antimicrobial drugs is well understood at the molecular level, a systems-level physiological response to antibiotics remains largely unexplored. This work considers fluctuation dynamics of both the chromosome and cytosol in Escherichia coli, and their response to sublethal treatments of a clinically important antibiotic, rifampicin. We precisely quantify the changes in dynamics of chromosomal loci and cytosolic aggregates (a rheovirus nonstructural protein known as μNS-GFP), measuring short time-scale displacements across several hours of drug exposure. To achieve this we develop an empirical method correcting for photo-bleaching and loci size effects. This procedure allows us to characterize the dynamic response to rifampicin in different growth conditions, including a customised microfluidic device. We find that sub-lethal doses of rifampicin cause a small but consistent increase in motility of both the chromosomal loci and cytosolic aggregates. Chromosomal and cytosolic responses are consistent with each other and between different growth conditions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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