Phylogenetic analysis of eukaryotic NEET proteins uncovers a link between a key gene duplication event and the evolution of vertebrates.

Autor: Inupakutika MA; Department of Biological Sciences, University of North Texas, Denton TX 76203, USA., Sengupta S; Department of Biological Sciences, University of North Texas, Denton TX 76203, USA., Nechushtai R; The Alexander Silberman Institute of Life Science, Hebrew University of Jerusalem, Edmond J. Safra Campus at Givat Ram, Jerusalem 91904, Israel., Jennings PA; Department of Chemistry &Biochemistry, University of California at San Diego, La Jolla, CA 92093, USA., Onuchic JN; Center for Theoretical Biological Physics and Department of Physics and Astronomy, Chemistry and Biosciences, 239 Brockman Hall, 6100 Main Street- MS-61, Rice University, Houston, TX 77005, USA., Azad RK; Department of Biological Sciences, University of North Texas, Denton TX 76203, USA.; Department of Mathematics, University of North Texas, Denton, TX 76203, USA., Padilla P; Department of Biological Sciences, University of North Texas, Denton TX 76203, USA., Mittler R; Department of Biological Sciences, University of North Texas, Denton TX 76203, USA.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2017 Feb 16; Vol. 7, pp. 42571. Date of Electronic Publication: 2017 Feb 16.
DOI: 10.1038/srep42571
Abstrakt: NEET proteins belong to a unique family of iron-sulfur proteins in which the 2Fe-2S cluster is coordinated by a CDGSH domain that is followed by the "NEET" motif. They are involved in the regulation of iron and reactive oxygen metabolism, and have been associated with the progression of diabetes, cancer, aging and neurodegenerative diseases. Despite their important biological functions, the evolution and diversification of eukaryotic NEET proteins are largely unknown. Here we used the three members of the human NEET protein family (CISD1, mitoNEET; CISD2, NAF-1 or Miner 1; and CISD3, Miner2) as our guides to conduct a phylogenetic analysis of eukaryotic NEET proteins and their evolution. Our findings identified the slime mold Dictyostelium discoideum's CISD proteins as the closest to the ancient archetype of eukaryotic NEET proteins. We further identified CISD3 homologs in fungi that were previously reported not to contain any NEET proteins, and revealed that plants lack homolog(s) of CISD3. Furthermore, our study suggests that the mammalian NEET proteins, mitoNEET (CISD1) and NAF-1 (CISD2), emerged via gene duplication around the origin of vertebrates. Our findings provide new insights into the classification and expansion of the NEET protein family, as well as offer clues to the diverged functions of the human mitoNEET and NAF-1 proteins.
Databáze: MEDLINE