Lycopene, resveratrol, vitamin C and FeSO 4 increase damage produced by pro-oxidant carcinogen 4-nitroquinoline-1-oxide in Drosophila melanogaster: Xenobiotic metabolism implications.

Autor: Dueñas-García IE; Genetic Toxicology Laboratory, Universidad Nacional Autónoma de México (UNAM), Los Barrios N° 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico., Heres-Pulido ME; Genetic Toxicology Laboratory, Universidad Nacional Autónoma de México (UNAM), Los Barrios N° 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico. Electronic address: heres@campus.iztacala.unam.mx., Arellano-Llamas MR; Genetic Toxicology Laboratory, Universidad Nacional Autónoma de México (UNAM), Los Barrios N° 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico., De la Cruz-Núñez J; Genetic Toxicology Laboratory, Universidad Nacional Autónoma de México (UNAM), Los Barrios N° 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico., Cisneros-Carrillo V; Genetic Toxicology Laboratory, Universidad Nacional Autónoma de México (UNAM), Los Barrios N° 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico., Palacios-López CS; Genetic Toxicology Laboratory, Universidad Nacional Autónoma de México (UNAM), Los Barrios N° 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico., Acosta-Anaya L; Genetic Toxicology Laboratory, Universidad Nacional Autónoma de México (UNAM), Los Barrios N° 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico., Santos-Cruz LF; Genetic Toxicology Laboratory, Universidad Nacional Autónoma de México (UNAM), Los Barrios N° 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico., Castañeda-Partida L; Genetic Toxicology Laboratory, Universidad Nacional Autónoma de México (UNAM), Los Barrios N° 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico., Durán-Díaz A; Mathematics, Biology, FES Iztacala, Universidad Nacional Autónoma de México (UNAM), Los Barrios N° 1, Los Reyes Iztacala, C.P. 54090, Tlalnepantla, Estado de México, Mexico.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2017 May; Vol. 103, pp. 233-245. Date of Electronic Publication: 2017 Feb 12.
DOI: 10.1016/j.fct.2017.02.007
Abstrakt: 4-nitroquinoline-1-oxide (4-NQO) is a pro-oxidant carcinogen bioactivated by xenobiotic metabolism (XM). We investigated if antioxidants lycopene [0.45, 0.9, 1.8 μM], resveratrol [11, 43, 172 μM], and vitamin C [5.6 mM] added or not with FeSO 4 [0.06 mM], modulate the genotoxicity of 4-NQO [2 mM] with the Drosophila wing spot test standard (ST) and high bioactivation (HB) crosses, with inducible and high levels of cytochromes P450, respectively. The genotoxicity of 4-NQO was higher when dissolved in an ethanol - acetone mixture. The antioxidants did not protect against 4-NQO in any of both crosses. In the ST cross, resveratrol [11 μM], vitamin C and FeSO 4 resulted in genotoxicity; the three antioxidants and FeSO 4 increased the damage of 4-NQO. In the HB cross, none of the antioxidants, neither FeSO 4 , were genotoxic. Only resveratrol [172 μM] + 4-NQO increased the genotoxic activity in both crosses. We concluded that the effects of the antioxidants, FeSO 4 and the modulation of 4-NQO were the result of the difference of Cyp450s levels, between the ST and HB crosses. We propose that the basal levels of the XM's enzymes in the ST cross interacted with a putative pro-oxidant activity of the compounds added to the pro-oxidant effects of 4-NQO.
(Copyright © 2017. Published by Elsevier Ltd.)
Databáze: MEDLINE
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