| Autor: |
Chatterjea A, LaPointe VL, Barradas A, Garritsen H, Yuan H, Renard A, van Blitterswijk CA, de Beor J; Department of Cell Biology-Inspired Tissue Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, 6229 ER Maastricht, The Netherlands.jan.deboer@maastrichtuniversity.nl. |
| Jazyk: |
angličtina |
| Zdroj: |
European cells & materials [Eur Cell Mater] 2017 Feb 15; Vol. 33, pp. 121-129. Date of Electronic Publication: 2017 Feb 15. |
| DOI: |
10.22203/eCM.v033a09 |
| Abstrakt: |
The amount of bone generated using current tissue engineering approaches is insufficient for many clinical applications. Previous in vitro studies suggest that culturing cells as 3D aggregates can enhance their osteogenic potential, but the effect on bone formation in vivo is unknown. Here, we use agarose wells to generate uniformly sized mesenchymal stromal cell (MSC) aggregates. When combined with calcium phosphate ceramic particles and a gel prepared from human platelet-rich plasma, we generated a tissue engineered construct which significantly improved in vivo bone forming capacity as compared to the conventional system of using single cells seeded directly on the ceramic surface. Histology demonstrated the reproducibility of this system, which was tested using cells from four different donors. In vitro studies established that MSC aggregation results in an up-regulation of osteogenic transcripts. And finally, the in vivo performance of the constructs was significantly diminished when unaggregated cells were used, indicating that cell aggregation is a potent trigger of in vivo bone formation by MSCs. Cell aggregation could thus be used to improve bone tissue engineering strategies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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